Skip to main content
Article thumbnail
Location of Repository

Fibroblast response is enhanced by poly(L-lactic acid) nanotopography edge density and proximity

By Keith R Milner and Christopher A Siedlecki

Abstract

The development of scaffolds for use in tissue engineering applications requires careful choice of macroscale properties, such as mechanical characteristics, porosity and biodegradation. The micro- and nano-scale properties of the scaffold surface are also an important design criterion as these influence cell adhesion, proliferation, and differentiation. The cellular response is known to be affected by surface topography but the mechanisms governing this remain unclear. Homogenous poly(L-lactic acid) was textured with surface nanotopographies by two-stage replication molding of heterogeneous demixed polymer films. Initial cell adhesion was improved on nanotextured surfaces compared with smooth controls, but subsequent cell density was significantly reduced on the roughest surfaces. Improvements in cell response were found to correlate with focal contact and actin microfilament development. Cell response was found to trend both with the surface density of topography edges and with inter-topography spacing, indicating possible roles for edges stimulating cell adhesion/proliferation or for spacing to modulate the ability of integrin-ligand bonds to cluster and form focal adhesions. This study furthers understanding of the geometric properties of surface nanotopographies that affect cellular response. It is hoped that identification of the mechanisms governing cell-topography interactions will allow rule-based design of biomaterial surface to engineer specific cellular responses

Topics: Original Research
Publisher: Dove Medical Press
OAI identifier: oai:pubmedcentral.nih.gov:2673967
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Citations

    1. (2006). Co-culture of bone marrow fi broblasts and endothelial cells on modifi ed polycaprolactone substrates for enhanced potentials in bone tissue engineering. Tissue Eng,
    2. (2003). Fibroblast reaction to island topography: changes in cytoskeleton and morphology with time.
    3. (2003). Nonadhesive nanotopography: fi broblast response to poly (n-butyl methacrylate)-poly (styrene) demixed surface features.
    4. (2002). Polymer-demixed nanotopography: Control of fi broblast spreading and proliferation.
    5. (2004). Rapid fi broblast adhesion to 27 nm high polymer demixed nano-topography.
    6. (1996). Surface topography and composition of deuterated polystyrene-poly (bromostyrene) blends.
    7. (1997). Topographical control of cells.

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.