Cesium Chloride Density Gradient Study of Some Modifications Induced by Ultraviolet Radiation on the Denatured DNA Molecule of Phage T2

Abstract

The behaviour of the modifications due to ultraviolet (UV) radiation on denatured phagic DNA has been investigated by looking at the changes of density in CsCl gradient, after irradiation with increasing doses of monochromatic light. The spectral range studied is 2300–2900 A, with a wavelength resolution Δλ = ±20 A. The observed effect is a gradual shift toward higher densities as the UV absorbed dose increases. The experimental results show an exponential law and the analysis of the action spectra indicates that thymine is the main factor responsible for the observed effect. An evaluation of the quantum yield for the thymine-dimers formation gives a constant value of (18±3)×10-3 quanta-1