Activity of Testosterone 5α-Reductase in Various Tissues of Human Skin

Abstract

In order to know the distribution of testosterone 5α-reductase activity in human skin, we developed a micro-method, in which we used 20–50 μg of various tissues microdissected from freeze-dried sections. The characteristics of this enzyme in the sebaceous gland are briefly described, as follows: the identified 5α-reduced metabolites are 5α-dihydrotestosterone, 5α-androstane-3β, 17β-diol and 5α-androstanedione; the optimal pH is about 7.5; and the apparent Km is approximately 2.4 × 10−5 M.The measurement of 5α-reductase activity of various components of the skin obtained from 7 men and 5 women revealed that the sweat gland (probably apocrine) in the axillary skin possessed the highest activity of 5α-reductase: the value was nearly 400 pmoles/mg dry weight/hr in the standardized condition. The sebaceous gland also showed a high activity of 85–261 pmoles/mg/hr. The hair follicles exhibited a significantly lower activity than the sebaceous gland. The enzyme activity was negligible in the epidermis, while it was detected in the dermis though the values determined were variable probably because of contamination with other components such as sweat glands and hair follicles.Thus, the present study demonstrates that the 5α-reductase activity is mainly located in the apocrine sweat gland and sebaceous gland. This suggests that 5α-reduction of testosterone is an important step in mediating the action of androgens in these tissues