Skip to main content
Article thumbnail
Location of Repository

Construction of a taste-blind medaka fish and quantitative assay of its preference–aversion behavior

By Y Aihara, A Yasuoka, S Iwamoto, Y Yoshida, T Misaka and K Abe


In vertebrates, the taste system provides information used in the regulation of food ingestion. In mammals, each cell group within the taste buds expresses either the T1R or the T2R taste receptor for preference–aversion discrimination. However, no such information is available regarding fish. We developed a novel system for quantitatively assaying taste preference–aversion in medaka fish. In this study, we prepared fluorescently labeled foods with fine cavities designed to retain tastants until they were bitten by the fish. The subjects were fed food containing a mixture of amino acids and inosine monophosphate (AN food), denatonium benzoate (DN food) or no tastant (NT food), and the amounts of ingested food were measured by fluorescence microscopy. Statistical analysis of the fluorescence intensities yielded quantitative measurements of AN food preference and DN food aversion. We then generated a transgenic fish expressing dominant-negative Gαi2 both in T1R-expressing and in T2R-expressing cells. The feeding assay revealed that the transgenic fish was unable to show a preference for AN food and an aversion to DN food. The assay system was useful for evaluating taste-blind behaviors, and the results indicate that the two taste signaling pathways conveying preferable and aversive taste information are conserved in fish as well as in mammals

Topics: Original Articles
Publisher: Blackwell Publishing Ltd
OAI identifier:
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles


    1. (2000). A novel family of mammalian taste receptors.
    2. (2004). A systematic genome-wide screen for mutations affecting organogenesis in Medaka, Oryzias latipes.
    3. (1998). Amino acid--activated channels in the catfish taste system.
    4. (2002). An amino-acid taste receptor.
    5. (2004). Behavioral responses of newly hatched zebrafish (Danio rerio) to amino acid chemostimulants.
    6. (1996). Brain and Behavior (2008) 7: 924–932 931 Taste-blind medaka and its feeding
    7. (2007). Characterization of ligands for fish taste receptors.
    8. (1977). Chemoreception in the Pigfish, Orthopristis chrysopterus: contribution of amino acids and betaine to stimulation of feeding behavior by various extracts.
    9. (2003). Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways.
    10. (2000). Comprehensive study on G protein a-subunits in taste bud cells, with special reference to the occurrence of Gai2 as a major Ga species.
    11. (1994). Consummatory feeding behavior to amino acids in intact and anosmic channel catfish Ictalurus punctatus.
    12. (2006). Feeding behaviour in some teleosts is triggered by single amino acids primarily through olfaction.
    13. (2005). Fish genomics and biology.
    14. (1995). Functional analysis of a dominant negative mutant of G_i2.
    15. (2001). G_ association and effector interaction selectivities of the divergent G_ subunit G_13.
    16. (1999). G_13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium.
    17. (1995). Gustatory control of feeding behavior in goldfish.
    18. (1975). Gustatory response in puffer-II. Single fiber analyses.
    19. (1979). Gustatory responses of eel palatine receptors to amino acids and carboxylic acids.
    20. (1992). Gustducin is a taste-cell-specific G protein closely related to the transducins.
    21. (2001). Mammalian sweet taste receptors.Cell 106,
    22. (2004). Neurogenesis. In
    23. (1967). Orientation by taste in fish of genus Ictalurus.
    24. (2004). Phospholipase C-beta 2 as a mammalian taste signaling marker is expressed in the multiple gustatory tissues of medaka fish, Oryzias latipes.
    25. (1993). Purification from Sf9 cells and characterization of recombinant Gq alpha and G11 alpha. Activation of purified phospholipase C isozymes by G alpha subunits.
    26. (1999). Putative mammalian taste receptors: a class of taste-specific GPCRs with distinct topographic selectivity.Cell 96,
    27. (1997). Quinine suppression of single facial taste fiber responses in the channel catfish.
    28. (2001). Regulation of phosphoinositide-specific phospholipase
    29. (1993). Regulation of purified subtypes of phosphatidylinositol-specific phospholipase C beta by G protein alpha and beta gamma subunits.
    30. (1992). Responses of single facial taste fibers in the channel catfish, Ictalurus punctatus, to amino acids.
    31. (1971). Structures and functions of sense of taste in catfish (Ictalurus natalis).
    32. (2000). T2Rs function as bitter taste receptors.
    33. (2004). Taste buds: development and evolution.
    34. (2003). Taste preferences in fishes.
    35. (1996). Taste sensitivity of common carp Cyprinus carpio to free amino acids and classical taste substances.
    36. (1992). Teleost gustation, in Fish chemoreception.
    37. (1994). The diversity of chemical stimulation in fish olfaction and gustation.
    38. (2005). The receptors and coding logic for bitter taste.
    39. (1996). Transduction of bitter and sweet taste by gustducin.
    40. (2007). Transgenic labeling of taste receptor cells in model fish under the control of the 50-upstream region of medaka phospholipase
    41. (2005). Two families of candidate taste receptors in fishes.
    42. (1985). Ultrastructure of mouse vallate taste buds. I. Taste cells and their associated synapses.

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.