Skip to main content
Article thumbnail
Location of Repository

The Carotenogenesis Pathway via the Isoprenoid-β-carotene Interference Approach in a New Strain of Dunaliella salina Isolated from Baja California Mexico

By J. Paniagua-Michel, Willian Capa-Robles, Jorge Olmos-Soto and Luis Enrique Gutierrez-Millan

Abstract

D. salina is one of the recognized natural sources to produce β-carotene, and an useful model for studying the role of inhibitors and enhancers of carotenogenesis. However there is little information in D. salina regarding whether the isoprenoid substrate can be influenced by stress factors (carotenogenic) or selective inhibitors which in turn may further contribute to elucidate the early steps of carotenogenesis and biosynthesis of β-carotene. In this study, Dunaliella salina (BC02) isolated from La Salina BC Mexico, was subjected to the method of isoprenoids-β-carotene interference in order to promote the interruption or accumulation of the programmed biosynthesis of carotenoids. When Carotenogenic and non-carotenogenic cells of D. salina BC02 were grown under photoautotrophic growth conditions in the presence of 200 µM fosmidomycin, carotenogenesis and the synthesis of β-carotene were interrupted after two days in cultured D. salina cells. This result is an indirect consequence of the inhibition of the synthesis of isoprenoids and activity of the recombinant DXR enzyme thereby preventing the conversion of 1-deoxy-D-xylulose 5-phosphate (DXP) to 2-C-methyl-D-erythritol (MEP) and consequently interrupts the early steps of carotenogenesis in D. salina. The effect at the level of proteins and RNA was not evident. Mevinolin treated D. salina cells exhibited carotenogenesis and β-carotene levels very similar to those of control cell cultures indicating that mevinolin not pursued any indirect action in the biosynthesis of isoprenoids and had no effect at the level of the HMG-CoA reductase, the key enzyme of the Ac/MVA pathway

Topics: Article
Publisher: Molecular Diversity Preservation International
OAI identifier: oai:pubmedcentral.nih.gov:2666888
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Citations

    1. (1993). A comparison of Lowry, Bradford and Smith protein assays using different protein standards and protein isolated from the marine diatom Thalassiosira
    2. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    3. (2001). Chlorophyta exclusively use the 1-deoxyxylulose 5-phosphate/2-C-methylerythritol 4-phosphate pathway for the biosynthesis of isoprenoids. Planta
    4. (2000). CO2 as main carbon source for isoprenoid biosynthesis via the mevalonate-independent methylerythritol 4-phosphate route in the marine diatoms Phaeodactylum tricornutum and Nitzschia ovalis. Phytochem.
    5. (2009). Crosstalk between cytosolic and plastidial pathways of isoprenoid biosynthesis in Arabidopsis thaliana.
    6. (1996). Desoxyribonucleic acid, protein and pigments during induced carotenogenesis in a new strain of Dunaliella salina (Chlorophyta isolated from a hipersaline coastal lagoon of Baja California. MSc Thesis,
    7. (1998). Distribution of the mevalonate and glyceraldehyde phosphate/pyruvate pathways for isoprenoid biosynthesis in unicellular algae and the cyanobacterium Synechocystis PCC 6714. Biochem
    8. (1987). Effect of irradiance and nutrient deficiency on the chemical composition of Dunaliella
    9. (2004). Effects of brassinazole, an inhibitor of brassinosteroid biosynthesis, on light and dark grown Chlorella vulgaris. Planta
    10. (1990). Effects of salinity increase on carotenoid accumulation in the green alga Dunaliella
    11. (2000). Fosmidomycin as an inhibitor of the non-mevalonate terpenoid pathway depresses synthesis of secondary carotenoids in flagellates of the green alga Haematococcus pluvialis.
    12. (1995). Growth of Dunaliella bardawil under carotenogenic conditions.
    13. (1991). identification, and quantification of carotenoids in fruits, vegetables and human plasma by high performance liquid chromatography.
    14. (2000). Isoprenoid biosynthesis: the evolution of two ancient and distinct pathways across genomes.
    15. (1995). Microalgae as source of pharmaceuticals and other biologically active compounds.
    16. (1989). Molecular cloning: a laboratory manual;
    17. (2002). Molecular identification of β-carotene hyper-producer strains of Dunaliella from saline environments using species-specific oligonucleotides.
    18. (1983). On the factors which determine massive beta-carotene accumulation in the halotolerant alga Dunaliella bardawil. Plant Physiol.
    19. (1983). On the Factors which Determine Massive β-Carotene Accumulation in the Halotolerant Alga Dunaliella bardawil. Plant Physiol.
    20. (1999). The 1-deoxy-D-xylulose-5-phosphate pathway of isoprenoid biosynthesis in plants. Annu Rev Plant Physiol Plant Mol Biol.
    21. (1999). The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher
    22. (1973). Transcription and translation of carotenoid synthesis in Chlamydomonas reinhardtii. Planta
    23. (2000). Young AJ Exposure of low irradiance favors the synthesis of 9-cis β, β-carotene in Dunaliella salina (Teod.) Plant Physiol.
    24. (1992). β-carotene biosynthesis.

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.