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Expression of four types of human tyrosine hydroxylase in COS cells

By Kazuto Kobayashi, Kazutoshi Kiuchi, Akira Ishii, Norio Kaneda, Yoshikazu Kurosawa, Keisuke Fujita and Toshiharu Nagatsu


AbstractAlternative splicing from a single gene produces four kinds of human tyrosine hydroxylase (types 1–4) which have structural diversity only in the N-terminal region. We attempted expression of the type 1–4 enzymes in COS cells and performed kinetic analyses. All had enzymatic activities. The Km values of the four types for L-tyrosine and 6-methyl-5,6,7,8-tetrahydropteridine were similar, although their relative homospecific activities were clearly different. The type 1 enzyme displayed the highest activity

Publisher: Published by Elsevier B.V.
Year: 1988
DOI identifier: 10.1016/0014-5793(88)80526-X
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