Skip to main content
Article thumbnail
Location of Repository

Specific and complete human genome amplification with improved yield achieved by phi29 DNA polymerase and a novel primer at elevated temperature

By Osama Alsmadi, Fadi Alkayal, Dorota Monies and Brian F Meyer
Topics: Short Report
Publisher: BioMed Central
OAI identifier:
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles


    1. (2004). A: Isothermal whole genome amplification from single and small numbers of cells: a new era for preimplantation genetic diagnosis of inherited disease. Mol Hum Reprod
    2. (1992). Arnheim N: Whole genome amplification from a single cell: implications for genetic analysis. Proc Natl Acad Sci USA
    3. (2002). Comprehensive human genome amplification using multiple displacement amplification. Proc Natl Acad Sci USA
    4. (2003). Egholm M: Whole genome amplification: abundant supplies of DNA from precious samples or clinical specimens. Trends Biotechnol
    5. (1991). EJ: High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene
    6. (2004). et al.: Parallel genotyping of over 10,000 SNPs using a one-primer assay on a high-density oligonucleotide array. Genome Res
    7. (2006). et al.: Singlecell chromosomal imbalances detection by array CGH. Nucleic Acids Res
    8. (1993). Fidelity of phi 29 DNA polymerase. Comparison between protein-primed initiation and DNA polymerization.
    9. (2005). JC: Cell-free cloning using phi29 DNA polymerase.
    10. (1991). Kunkel TA: DNA polymerase fidelity and the polymerase chain reaction.
    11. (2005). L: Genome amplification of single sperm using multiple displacement amplification. Nucleic Acids Res
    12. (2005). L: Genome-wide single-nucleotide polymorphism arrays demonstrate high fidelity of multiple displacementbased whole-genome amplification. Electrophoresis
    13. (2001). Lasken RS: Rapid amplification of plasmid and phage DNA using Phi 29 DNA polymerase and multiply-primed rolling circle amplification. Genome Res
    14. (2003). Lasken RS: Unbiased whole-genome amplification directly from clinical samples. Genome Res
    15. (2006). Leamon JH: Assessment of whole genome amplification-induced bias through high-throughput, massively parallel whole genome sequencing.
    16. (2004). Makrigiorgos GM: Balanced-PCR amplification allows unbiased identification of genomic copy changes in minute cell and tissue samples. Nucleic Acids Res
    17. (1975). On the stimulation of viral DNA polymerase activity by nonionic detergent. Biochemistry
    18. (2006). Optimization and evaluation of single-cell whole-genome multiple displacement amplification. Hum Mutat
    19. (2007). Quake SR: Nanoliter reactors improve multiple displacement amplification of genomes from single cells. PLoS Genet
    20. (1996). SF: Whole genome amplification using a degenerate oligonucleotide primer allows hundreds of genotypes to be performed on less than one nanogram of genomic DNA.
    21. (2006). Syvanen AC: Multiple displacement amplification to create a long-lasting source of DNA for genetic studies. Hum Mutat
    22. (2003). Syvanen AC: Quantitative evaluation by minisequencing and microarrays reveals accurate multiplexed SNP genotyping of whole genome amplified DNA. Nucleic Acids Res
    23. (2006). Towards the analysis of the genomes of single cells: further characterisation of the multiple displacement amplification. Gene
    24. (1992). Tunnacliffe A: Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer. Genomics
    25. (2003). Whole genome analysis of genetic alterations in small DNA samples using hyperbranched strand displacement amplification and arrayCGH. Genome Res

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.