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Heterochromatin Protein 1 (HP1) Proteins Do Not Drive Pericentromeric Cohesin Enrichment in Human Cells

By Ángel Serrano, Miriam Rodríguez-Corsino and Ana Losada

Abstract

Sister chromatid cohesion mediated by cohesin is essential for accurate chromosome segregation. Classical studies suggest that heterochromatin promotes cohesion, but whether this happens through regulation of cohesin remains to be determined. Heterochromatin protein 1 (HP1) is a major component of heterochromatin. In fission yeast, the HP1 homologue Swi6 interacts with cohesin and is required for proper targeting and/or stabilization of cohesin at the centromeric region. To test whether this pathway is conserved in human cells, we have examined the behavior of cohesin in cells in which the levels of HP1 alpha, beta or gamma (the three HP1 proteins present in mammalian organisms) have been reduced by siRNA. We have also studied the consequences of treating human cells with drugs that change the histone modification profile of heterochromatin and thereby affect HP1 localization. Our results show no evidence for a requirement of HP1 proteins for either loading of bulk cohesin onto chromatin in interphase or retention of cohesin at pericentric heterochromatin in mitosis. However, depletion of HP1gamma leads to defects in mitotic progression

Topics: Research Article
Publisher: Public Library of Science
OAI identifier: oai:pubmedcentral.nih.gov:2662427
Provided by: PubMed Central
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    Citations

    1. (2004). A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1.
    2. Allshire RC (2004) Loss of Dicer fowls up centromeres.
    3. (2008). Association of cohesin and Nipped-B with transcriptionally active regions of the Drosophila melanogaster genome.
    4. (2005). Characterization of Dicer-deficient murine embryonic stem cells.
    5. (2000). Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis.
    6. (2003). Chromosomal distribution of heterochromatin protein 1 (HP1) in Drosophila: a cytological map of euchromatic HP1 binding sites.
    7. (2008). Cohesin mediates transcriptional insulation by CCCTC-binding factor.
    8. (2002). Cohesin release is required for sister chromatid resolution, but not for condensin-mediated compaction, at the onset of mitosis.
    9. (2004). Cohesin relocation from sites of chromosomal loading to places of convergent transcription.
    10. (1998). Cohesion between sister chromatids must be established during DNA replication.
    11. (2004). Dicer is essential for formation of the heterochromatin structure in vertebrate cells.
    12. (2005). Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing.
    13. (2003). Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cells.
    14. (2005). Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2. PLoS
    15. FJ 3rd (2005) The mammalian heterochromatin protein 1 binds diverse nuclear proteins through a common motif that targets the chromoshadow domain.
    16. (2005). Functional contribution of Pds5 to cohesin-mediated cohesion in human cells and Xenopus egg extracts.
    17. (1992). Functional elements in Drosophila melanogaster heterochromatin.
    18. (2004). Genome-wide mapping of the cohesin complex in the yeast Saccharomyces cerevisiae.
    19. (2007). H3K9 methylation and RNA interference regulate nucleolar organization and repeated DNA stability.
    20. (2008). Heterochromatin links to centromeric protection by recruiting shugoshin.
    21. (1995). Heterochromatin protein 1 is required for correct chromosome segregation in Drosophila embryos.
    22. (2004). HP1 and the dynamics of heterochromatin maintenance.
    23. (2003). HP1 complexes and heterochromatin assembly.
    24. (2005). HP1 modulates the transcription of cell-cycle regulators in Drosophila melanogaster.
    25. (2008). HP1-beta is required for development of the cerebral neocortex and neuromuscular junctions.
    26. (2008). HP1: a functionally multifaceted protein.
    27. (2001). Human chromatid cohesin component hRad21 is phosphorylated in M phase and associated with metaphase centromeres.
    28. (2006). Human Scc4 is required for cohesin binding to chromatin, sister-chromatid cohesion, and mitotic progression.
    29. (2006). Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase.
    30. (2000). Identification and characterization of SA/Scc3p subunits in the Xenopus and human cohesin complexes.
    31. (1986). Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene.
    32. Imhof A (2007) HP1 binding to chromatin methylated at H3K9 is enhanced by auxiliary factors.
    33. (1999). Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells.
    34. (2001). Loss of the Suv39h histone methyltransferases impairs mammalian heterochromatin and genome stability.
    35. (2001). Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.
    36. (2004). Mouse centric and pericentric satellite repeats form distinct functional heterochromatin.
    37. (2007). Noncoding RNAs and gene silencing.
    38. (2008). Perturbation of HP1 localization and chromatin binding ability causes defects in sister-chromatid cohesion.
    39. (2007). PHB2 protects sister-chromatid cohesion in mitosis.
    40. (2002). Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast.
    41. (2006). Regulation of mitotic chromosome cohesion by haspin and aurora B.
    42. (2001). Requirement of heterochromatin for cohesion at centromeres.
    43. (2001). Reversible disruption of pericentric heterochromatin and centromere function by inhibiting deacetylases.
    44. (2001). Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain.
    45. (2006). Separase: a universal trigger for sister chromatid disjunction but not chromosome cycle progression.
    46. (1981). Sequence of centromere separation: an analysis of mitotic chromosomes from long-term cultures of Potorus cells.
    47. (2005). Sex-specific role of Drosophila melanogaster HP1 in regulating chromatin structure and gene transcription.
    48. (2007). Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres.
    49. (2008). Sister chromatid cohesion: a simple concept with a complex reality.
    50. (1992). Sister chromatid separation in frog egg extracts requires DNA topoisomerase II activity during anaphase.
    51. (1994). Sister-chromatid cohesion in mitosis and meiosis.
    52. (2008). The cohesin complex and its roles in chromosome biology.
    53. (2005). The diverse functions of histone lysine methylation.
    54. (2000). The Drosophila RAD21 cohesin persists at the centromere region in mitosis.
    55. (2006). The Heterochromatin Protein 1 family.
    56. (1998). The heterochromatin protein 1 prevents telomere fusions in Drosophila.
    57. (2004). The kinetochore is an enhancer of pericentric cohesin binding.
    58. (2008). The regulation of sister chromatid cohesion.
    59. (1991). The spindle is required for the process of sister chromatid separation in Drosophila neuroblasts.
    60. (2008). The Suv39hHP1 histone methylation pathway is dispensable for enrichment and protection of cohesin at centromeres in mammalian cells.
    61. (1993). Topoisomerase II inhibition prevents anaphase chromatid segregation in mammalian cells independently of the generation of DNA strand breaks.
    62. (2000). Two distinct pathways remove mammalian cohesin from chromosome arms in prophase and from centromeres in anaphase.
    63. (2008). Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells.
    64. (2006). Wapl controls the dynamic association of cohesin with chromatin.

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