journal article
oaidoi:10.1016/j.scr.2016.05.010

A human MIXL1 green fluorescent protein reporter embryonic stem cell line engineered using TALEN-based genome editing

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Publication date
31 July 2016
Publisher
The Author(s). Published by Elsevier B.V.
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    Abstract

    AbstractWe have generated a MIXL1-eGFP reporter human embryonic stem cell (hESC) line using TALEN-based genome engineering. This line accurately traces endogenous MIXL1 expression via an eGFP reporter to mesendodermal precursor cells. The utility of the MIXL1-eGFP reporter hESC line lies in the prospective isolation, lineage tracing, and developmental and mechanistic studies of MIXL1+ cell populations

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