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Elucidating the selenium and arsenic metabolic pathways following exposure to the non-hyperaccumulating Chlorophytum comosum, spider plant

By Scott E. Afton, Brittany Catron and Joseph A. Caruso

Abstract

Although many studies have investigated the metabolism of selenium and arsenic in hyperaccumulating plants for phytoremediation purposes, few have explored non-hyperaccumulating plants as a model for general contaminant exposure to plants. In addition, the result of simultaneous supplementation with selenium and arsenic has not been investigated in plants. In this study, Chlorophytum comosum, commonly known as the spider plant, was used to investigate the metabolism of selenium and arsenic after single and simultaneous supplementation. Size exclusion and ion-pairing reversed phase liquid chromatography were coupled to an inductively coupled plasma mass spectrometer to obtain putative metabolic information of the selenium and arsenic species in C. comosum after a mild aqueous extraction. The chromatographic results depict that selenium and arsenic species were sequestered in the roots and generally conserved upon translocation to the leaves. The data suggest that selenium was directly absorbed by C. comosum roots when supplemented with SeVI, but a combination of passive and direct absorption occurred when supplemented with SeIV due to the partial oxidation of SeIV to SeVI in the rhizosphere. Higher molecular weight selenium species were more prevalent in the roots of plants supplemented with SeIV, but in the leaves of plants supplemented with SeVI due to an increased translocation rate. When supplemented as AsIII, arsenic is proposed to be passively absorbed as AsIII and partially oxidized to AsV in the plant root. Although total elemental analysis demonstrates a selenium and arsenic antagonism, a compound containing selenium and arsenic was not present in the general aqueous extract of the plant

Topics: Research Papers
Publisher: Oxford University Press
OAI identifier: oai:pubmedcentral.nih.gov:2657536
Provided by: PubMed Central
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    Citations

    1. (2001). A fern that hyperaccumulates arsenic.
    2. (2000). A metabolic link between arsenite and selenite: the seleno-bis(S-glutathionyl) arsinium ion.
    3. (2007). A study of Se-Hg antagonism in Glycine max (soybean) roots by size exclusion and reversed phase HPLC-ICPMS.
    4. (2001). An analysis of cancer prevention by selenium.
    5. (2004). Arsenic and its speciation analysis using high-performance liquid chromatography and inductively coupled plasma mass spectrometry.
    6. (2007). Arsenic in the environment: biology and chemistry.
    7. (1982). Arsenic metabolism in freshwater and terrestrial plants.
    8. (1969). Aspects of selenium metabolism in higher plants.
    9. (1999). Bacterial respiration of arsenic and selenium.
    10. (1999). Biogeochemical processes in the rhizosphere: role in phytoremediation of metal-polluted soils.
    11. (2008). Can we trust mass spectrometry for determination of arsenic peptides in plants: comparison of LC-ICP-MS and LC-ES-MS/ICP-MS with XANES/EXAFS in analysis of Thunbergia alata.
    12. (1981). Changes of pH across the rhizosphere induced by roots.
    13. (2006). Comparing a selenium accumulator plant (Brassica juncea)t oan o n -accumulator plant (Helianthus annuus) to investigate selenium-containing proteins.
    14. (2006). Detoxification/ defense mechanisms in metal-exposed plants.
    15. (2005). Distribution of elements binding to molecules with different molecular weights in aqueous extract of Antarctic krill by size-exclusion chromatography coupled with inductively coupled plasma mass spectrometry.
    16. (2004). Effects of arsenic species and phosphorus on arsenic absorption, arsenate reduction and thiol formation in excised parts of Pteris vittata L.
    17. (2008). Effects of nutrients on arsenic accumulation by arsenic hyperaccumulator Pteris vittata L.
    18. (1940). Further studies on the effectiveness of arsenic in preventing selenium poisoning.
    19. (2006). Localization and speciation of selenium and mercury in Brassica juncea: implications for Se-Hg antagonism.
    20. (2006). Occurrence of arsenic contamination in Canada: sources, behavior and distribution.
    21. (1982). Plant-induced changes in the rhizosphere of rape (Brassica napus var. Emerald) seedlings.
    22. (2000). Reduction and coordination of arsenic in Indian mustard.
    23. (1987). Reduction of selenate to selenide by sulphate-respiring bacteria: experiments with cell suspensions and estuarine sediments.
    24. Selenium arsenic metabolic pathways
    25. (2005). Selenium fractionation and speciation in rocks, soils, waters and plants in polluted surface mine environment.
    26. (2000). Selenium in higher plants.
    27. (2006). Selenium speciation analysis using inductively coupled plasma-mass spectrometry.
    28. (2006). Selenium speciation studies in Se-enriched chives (Allium schoenoprasum)b y HPLC-ICP-MS.
    29. (2007). Selenium transformation studies during broccoli (Brassica oleracea) growing process by liquid chromatography-inductively coupled plasma mass spectrometry (LC-ICP-MS).
    30. (1996). Selenium uptake by sulphur-accumulating bacteria.
    31. (2001). Selenium, selenoproteins and human health: a review.
    32. (2008). Simultaneous characterization of selenium and arsenic analytes via ion-pairing reversed phase chromatography with inductively coupled plasma and electrospray ionization ion trap mass spectrometry for detection.
    33. (2006). Spatial imaging, speciation, and quantification of selenium in the hyperaccumulator plants Astragalus bisulcatus and Stanleya pinnata.
    34. (2006). Study of phytochelatins and other related thiols as complexing biomolecules of As and Cd in wild type and genetically modified Brassica juncea plants.
    35. (2008). Synthesis of selenocysteine and selenomethionine derivatives from sulphur-containing amino acids.
    36. (2008). The identification and quantification of arsenic-induced phytochelatins: comparison between plants with varying As sensitivities.
    37. (1938). Water-culture method for growing plants without soil.

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