Skip to main content
Article thumbnail
Location of Repository

Bacterial artificial chromosomes improve recombinant protein production in mammalian cells

By Leander Blaas, Monica Musteanu, Robert Eferl, Anton Bauer and Emilio Casanova
Topics: Methodology Article
Publisher: BioMed Central
OAI identifier:
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles


    1. (1998). AF: A new logic for DNA engineering using recombination in Escherichia coli. Nature genetics
    2. (2007). BF: An efficient and targeted gene integration system for high-level antibody expression. Journal of immunological methods
    3. (2007). Casanova E: PhiC31-mediated cassette exchange into a bacterial artificial chromosome. BioTechniques
    4. (2001). DL: Recombineering: a powerful new tool for mouse functional genomics. Nature reviews
    5. (1991). Efficient selection for highexpression transfectants with a novel eukaryotic vector. Gene
    6. (1999). Generalized lacZ expression with the ROSA26 Cre reporter strain. Nature genetics
    7. (1997). Homologous recombination based modification in Escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome. Nature biotechnology
    8. (2003). Identification of anti-repressor elements that confer high and stable protein production in mammalian cells. Nature biotechnology
    9. (2001). Montoliu L: Size matters: use of YACs, BACs and PACs in transgenic animals. Transgenic research
    10. (2004). Production of recombinant protein therapeutics in cultivated mammalian cells. Nature biotechnology
    11. (1990). Ruker F: Nucleotide sequences of the cDNAs encoding the V-regions of H- and L-chains of a human monoclonal antibody specific to HIV-1-gp41. Nucleic acids research
    12. (2005). Villereal ML: Gene expression profiles in HEK-293 cells with low or high store-operated calcium entry: can regulatory as well as regulated genes be identified? Physiological genomics

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.