The Sequence of theOrgyia pseudotsugataMultinucleocapsid Nuclear Polyhedrosis Virus Genome

Abstract

AbstractThe nucleotide sequence of theOrgyia pseudotsugatamultinucleocapsid nuclear polyhedrosis virus (OpMNPV) genome was completed and analyzed. It is composed of 131,990 bases with a G + C content of 55% and contains 152 putative genes of 150 nucleotides or greater. Major differences in gene content and arrangement between OpMNPV and theAutographa californicaMNPV were found. These include the presence in OpMNPV of three completeiapgene homologs, two conotoxin gene homologs, two protein tyrosine phosphatase homologs, and genes encoding homologs of dUTPase and the large and small subunits of ribonucleotide reductase. Seven major intergenic repeated regions were identified. Five of these are homologous regions that are related to similar regions from other baculoviruses