Antioxidant effect of mesalazine in the experimental colitis model induced by acetic acid

Abstract

AbstractIntroductionInflammatory bowel disease (IBD) is characterized by a chronic inflammation of the gastrointestinal tract, without specific cause or pathogen.ObjectiveThe effect of mesalazine in a colitis model induced by acetic acid (AA) was evaluated.MethodsWe used 40 Wistar rats, ±350g, divided into 4 groups: control (CO); control+mesalazine (CO+M); colitis (CL) and colitis+M (CL+M) at 24 and 48h of treatment. The animals received the substances by an intracolonic enema of AA 4% and treatment with mesalazine PO 20mg/kg after colitis induction.ResultsMesalazine reduced tissue damage in the gut, normalized sphincter anal pressure levels and decreased lipid peroxidation, metabolites of nitric oxide and iNOS and NF-kB expression in the treated groups in both treatment time points (24 and 48h), as well as the activity of antioxidant enzymes.ConclusionMesalazine was effective in reducing tissue damage and oxidative and inflammatory damage, restored antioxidant capacity and increased anal sphincter pressure levels, possibly due to its antioxidant effect