Determination of Fat-soluble Vitamins in Food and Pharmaceutical Supplements Using Packed-fiber Solid Phase Extraction (PFSPE) for Sample Preconcentration/ Clean-up

Abstract

AbstractA novel and rapid method has been developed for separation and preconcentration of fat-soluble vitamins using packed-fiber solid phase extraction from complex matrices including food and pharmaceutical supplements. The extraction time is reduced to 5minutes which considerably shorter than in conventional methods. Other advantages are the reduction of manual manipulations leading to lower labour costs and reduced consumption of organic solvents in the sample preparation step.The analytical parameters including linearity, r > 0.9990; limit of detection, 1.0colong/mL,0.1colong/mL,1.0colong/mL and 0.1colong/mL for retinyl esters, cholecalciferol, tocopherol acetate and phylloquinone respectively; precision of the method, R.S.D. < 5% and recovery more than 90%, show that the method is appropriate for measuring fat-soluble vitamins in food and pharmaceutical supplements sample preparations