In Vivo Results with a New Device for Ultrasonic Monitoring of Pig Skin Cryosurgery: The Echographic Cryoprobe

Abstract

One of the main difficulties encountered in cryosurgery is the uncertainty in the extent and depth of the tissue effectively treated during the freezing process. The objective of this study was to evaluate in vivo ultrasonic control of skin cryosurgery using a new echographic cryoprobe. An echographic cryoprobe, developed specifically for dermatology applications, combines a high-frequency (20 MHz) miniature ultrasonic transducer and a N2O-driven closed cryoprobe. Knowledge of the ultrasound velocity of frozen skin is a prerequisite for monitoring the iceball formation kinetics. Therefore, in a first study, we estimated the ultrasound velocity of frozen skin specimens. In a second step, the operation of the echographic cryoprobe was assessed, under in vivo conditions similar to those used in human therapeutics, on normal skin of three female “Large-White” pigs under anesthesia. The mean value of ultrasound velocity of frozen skin obtained by pooling the data from all the skin specimens included in this study was 2865 ± 170 m per s. The average rates of growth (10–2 mm per s) of the iceballs were found to be 12.2 ± 1.0 (pig 1), 9.0 ± 1.0 (pig 2), and 8.4 ± 0.9 (pig 3). The echographic cryoprobe had a built-in high-frequency ultrasonic transducer that served two functions. It enabled in vivo real-time monitoring of depth penetration of the iceball and it gave important feedback to the operator or to the console relating to the rate of growth of the iceball. Automatic (i.e., operator-independent) detection of the echo signal from the freezing front and calculation of the depth penetration of the iceball was possible. Key word: ultrasound. J Invest Dermatol 111:314–319 199