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Spiroplasma taxonomy and identification of the sex ratio organisms: can they be cultivated?

By D. L. Williamson, T. Steiner and G. J. McGarrity


The spiroplasmas that occur naturally in several species of Drosophila were the first spiroplasmas ever observed, even though their discoverers, D.F. Poulson and B. Sakaguchi, in 1961 described them as being "treponema-like spirochetes." These Drosophila spiroplasmas are transovarially, or maternally, transmitted by infected females whose progenies are composed entirely of females. A more recently discovered Drosophila spiroplasma found in flies originating in Ito, Japan, is also maternally inherited but does not result in the elimination of males from the progeny of infected females. In spite of their early discovery, their high numerical density in the hemolymph of infected females (10(6)-10(7)/microliters), and numerous attempts at in vitro cultivation, they remain prime examples of non-cultivable spiroplasmas. It is the purpose of this paper to recount some of the approaches used in attempts at their cultivation

Topics: Research Article
Publisher: Yale Journal of Biology and Medicine
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Provided by: PubMed Central

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  1. al: Non-male-killing spiroplasmas in Drosophila hydei.
  2. (1967). Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L). Current Sci 36:506-508,
  3. (1972). Cell lines derived from late embryonic stages of Drosophila melanogaster.
  4. (1980). Cloning of hybridomas. Cloning in semisolid agarose. In Monoclonal Antibodies. Edited by RH Kennett,
  5. (1977). Comparative growth and primary isolation ofspiroplasmas in media based on insect tissue culture formulations. Phytopathol 68:739-746,
  6. (1979). DF: Sex ratio organisms (spiroplasmas) of Drosophila. In The Mycoplasmas. Vol 3. Edited by RF Whitcomb, JG Tully.
  7. Differentiation of larval Drosophila eye-antennal discs in vitro.
  8. (1981). DM: Cultivation and partial characterization of spiroplasmas in cell cultures. Infect Immun 35:296-304,
  9. (1981). Drosophila cells and cell lines.
  10. (1962). Establishment of four strains of cells from insect tissues grown in vitro.
  11. (1974). Gingeras TR, Kambysellis MP: Yolk proteins in Drosophila: Identification and site of synthesis.
  12. (1976). Influence of amino acids, mammalian serum, and osmotic pressure on the proliferation of Drosophila cell lines.
  13. (1975). Insect cell culture: Improved media and methods for initiating attached cell lines from the Lepidoptera. In Vitro 11:369-378,
  14. Isolement et culture in vitro des mycoplasmes associes au 'Stubborn' des agrumes et leur observation au microscope electronique.
  15. (1965). Kinetic studies of "sex-ratio" spirochetes in Drosophila melanogaster Meigen females.
  16. Maintenance of imaginal discs ofDrosophila melanogaster in chemically defined media.
  17. (1961). Nature of "sex-ratio" agent in Drosophila. Science 133:1489-1490,
  18. Ohanessian A: In vitro culture of Drosophila melanogaster cells.
  19. (1977). Pathogenic mycoplasmas: Cultivation and vertebrate pathogenecity of a new spiroplasma.
  20. (1977). Sang JH: Improved medium for culture of Drosophila embryonic cells. Dros Inf Serv 52:161,
  21. Spirochete-mediated abnormal sex-ratio (SR) condition in Drosophila: A second virus associated with spirochetes and its use in the study of the SR condition.
  22. (1975). TA: A simple medium for the isolation and cultivation of corn stunt spiroplasma.
  23. (1979). The spiroplasma deformation test, a new serological method. Current Microbiol 1:203-207,
  24. (1975). Two tissue culture media for production oflepidopteran cells and nuclear polyhedrosis viruses.
  25. (1018). Whitcomb RF: Plant mycoplasmas: A cultivable spiroplasma causes corn disease.

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