Evaluation of Pseudomonas aeruiginosa Biofilm Formation using Quartz Tuning Forks as Impedance Sensors

Abstract

AbstractShape of metallisation on Quartz Tuning Forks (QTFs) allowed to use them as impedance sensors for monitoring biofilm formation of Psedomonas aeruginosa strains. Modelling using electrical equivalent circuits was used. It allowed to detect which electrical properties of sensor change with biofilm formation. Characteristic frequencies of 20Hz and 1MHz were found as suitable for simple detection of biofilm state. Resistance measured at 1MHz allowed to detect changes in media conductivity caused by biofilm growth. It was in accordance with results obtained using another methods. In addition, the conductance at 20Hz was suitable for detection of biofilm destruction caused by the lack of nutrients