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Spatial configuration of the chicken α-globin gene domain: immature and active chromatin hubs

By Alexey A. Gavrilov and Sergey V. Razin


The spatial configuration of the chicken α-globin gene domain in erythroid and lymphoid cells was studied by using the Chromosome Conformation Capture (3C) approach. Real-time PCR with TaqMan probes was employed to estimate the frequencies of cross-linking of different restriction fragments within the domain. In differentiated cultured erythroblasts and in 10-day chick embryo erythrocytes expressing ‘adult’ αA and αD globin genes the following elements of the domain were found to form an ‘active’ chromatin hub: upstream Major Regulatory Element (MRE), −9 kb upstream DNase I hypersensitive site (DHS), −4 kb upstream CpG island, αD gene promoter and the downstream enhancer. The αA gene promoter was not present in the ‘active’ chromatin hub although the level of αA gene transcription exceeded that of the αD gene. Formation of the ‘active’ chromatin hub was preceded by the assembly of multiple incomplete hubs containing MRE in combination with either −9 kb DHS or other regulatory elements of the domain. These incomplete chromatin hubs were present in proliferating cultured erythroblasts which did not express globin genes. In lymphoid cells only the interaction between the αD promoter and the CpG island was detected

Topics: Genomics
Publisher: Oxford University Press
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Provided by: PubMed Central
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    1. (2004). 3C technology: analyzing the spatial organization of genomic loci in vivo.
    2. (2006). A CTCF-dependent silencer located in the differentially methylated area may regulate expression of a housekeeping gene overlapping a tissue-specific gene domain.
    3. (1992). A transcription-dependent DNase I-hypersensitive site in a far upstream segment of the chicken a-globin gene domain coincides with a matrix attachment region.
    4. (2006). Active chromatin hub of the mouse alphaglobin locus forms in a transcription factory of clustered housekeeping genes.
    5. (1991). Adult chicken a-globin gene expression in transfected QT6 quail cells: evidence for a negative regulatory element in the a D globin region.
    6. (1981). AlphaGlobin-gene switching during the development of chicken embryos: expression and chromosome structure.
    7. (1995). Analysis of the replication direction through the domain of a-globin-encoding genes.
    8. (2005). Annotation of cis-regulatory elements by identification, subclassification, and functional assessment of multispecies conserved sequences.
    9. (2007). Beta-globin active chromatin Hub formation in differentiating erythroid cells and in p45 NF-E2 knock-out mice.
    10. (2002). Capturing chromosome conformation.
    11. (1994). Characterization of the chromatin structure in the upstream area of the chicken a-globin gene domain.
    12. (1979). Chicken hematopoietic cells transformed by seven strains of defective avian leukemia viruses display three distinct phenotypes of differentiation.
    13. (2001). Chromatin domains and regulation of gene expression: familiar and enigmatic clusters of chicken globin genes.
    14. (1998). Chromatin interaction mechanism of transcriptional control in vivo.
    15. (2004). Comparative analysis of the alpha-like globin clusters in mouse, rat, and human chromosomes indicates a mechanism underlying breaks in conserved synteny.
    16. (2001). Comparative genome analysis delimits a chromosomal domain and identifies key regulatory elements in the alpha globin cluster.
    17. (1995). Conservation of position and sequence of a novel, widely expressed gene containing the major human alpha-globin regulatory element.
    18. (2006). CTCF mediates long-range chromatin looping and local histone modification in the beta-globin locus.
    19. (2004). CTCF-dependent enhancer blockers at the upstream region of the chicken alpha-globin gene domain.
    20. (1979). Erythroblast cell lines transformed by a temperature sensitive mutant of avian erythroblastosis virus. A model system to study erythroid differentiation in vitro.
    21. (1973). Erythropoiesis in the developing chick embryo.
    22. (2000). Extensive methylation of a part of the CpG island located 3.0-4.5 Kbp upstream to the chicken alpha-globin gene cluster may contribute to silencing the globin genes in non-erythroid cells.
    23. (1999). Functional analysis of DNA sequences located within a cluster of DNase I hypersensitive sites colocalising with MAR element at the upstream border of the chicken a-globin gene domain.
    24. (2006). Genome-wide scanning of HoxB1-associated loci in mouse ES cells using an open-ended Chromosome Conformation Capture methodology.
    25. (2006). How transcriptional and epigenetic programmes are played out on an individual mammalian gene cluster during lineage commitment and differentiation.
    26. (1989). Identification and characterization of a chicken a-globin enhancer.
    27. (1991). Induction of differentiation of avian erythroblastosis virus-transformed erythroblasts by the protein kinase inhibitor H7: analysis of the transcription factor EF1.
    28. (1985). K562 human erythroleukemia cells demonstrate commitment.
    29. (2007). Long-range chromosomal interactions regulate the timing of the transition between poised and active gene expression.
    30. (2005). Long-range interactions between three transcriptional enhancers, active Vkappa gene promoters, and a 3’ boundary sequence spanning 46 kilobases.
    31. (2002). Looping and interaction between hypersensitive sites in the active beta-globin locus.
    32. (2007). Mechanisms controlling activation of the alpha-globin gene domain in chicken erythroid cells.
    33. (2002). Methylation of alpha-type embryonic globin gene alpha pi represses transcription in primary erythroid cells.
    34. (2005). Proximity among distant regulatory elements at the beta-globin locus requires GATA-1 and FOG-1.
    35. (2007). Quantitative analysis of chromosome conformation capture assays (3C-qPCR).
    36. (1986). Replication origins are attached to the nuclear skeleton.
    37. (2003). Spatial organization of gene expression: the active chromatin hub.
    38. (1974). Structural studies on chick embryonic hemoglobins.
    39. (2004). The 33kb transcript of the chicken alpha-globin gene domain is part of the nuclear matrix.
    40. (2003). The beta-globin nuclear compartment in development and erythroid differentiation.
    41. (1983). The nucleotide sequence of the adult chicken a-globin genes.
    42. (1995). Transcription complex stability and chromatin dynamics in vivo.

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