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Measurement of functional microcirculatory geometry and velocity distributions using automated image analysis

By J. G. G. Dobbe, G. J. Streekstra, B. Atasever, R. van Zijderveld and C. Ince

Abstract

This study describes a new method for analyzing microcirculatory videos. It introduces algorithms for quantitative assessment of vessel length, diameter, the functional microcirculatory density distribution and red blood-cell (RBC) velocity in individual vessels as well as its distribution. The technique was validated and compared to commercial software. The method was applied to the sublingual microcirculation in a healthy volunteer and in a patient during cardiac surgery. Analysis time was reduced from hours to minutes compared to previous methods requiring manual vessel identification. Vessel diameter was detected with high accuracy (>80%, d > 3 pixels). Capillary length was estimated within 5 pixels accuracy. Velocity estimation was very accurate (>95%) in the range [2.5, 1,000] pixels/s. RBC velocity was reduced by 70% during the first 10 s of cardiac luxation. The present method has been shown to be fast and accurate and provides increased insight into the functional properties of the microcirculation

Topics: Original Article
Publisher: Springer-Verlag
OAI identifier: oai:pubmedcentral.nih.gov:2441502
Provided by: PubMed Central
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    1. (1986). A computational approach to edge detection.
    2. (2005). A new video image analysis system to study red blood cell dynamics and oxygenation in capillary networks.
    3. (1988). A versatile video image analysis system for microcirculatory research.
    4. (2001). Abnormal microcirculation in brain tumours during surgery.
    5. (1998). An unbiased detector of curvilinear structures.
    6. (2003). Anisotropy. In: Ter Haar Romeny BM (ed) Front-end vision and multi-scale image analysis: multiscale computer vision theory and applications,
    7. (1992). Application of image analysis for evaluation of red blood cell dynamics in capillaries.
    8. (2004). Blood transfusion increases functional capillary density in de skin of anemic preterm infants.
    9. (1997). Cap Image—ein neuartiges computerunterstu ¨tztes Videobildanalysesystem fu ¨r die dynamische Kapillarmikroskopie.
    10. (1995). Comparing methods of measurement: why plotting difference against standard method is misleading.
    11. (2001). Comparison of OPS imaging and conventional capillary microscopy to study the human microcirculation.
    12. (2002). Comparison of the new OPS imaging technique with intravital microscopy: analysis of the colon microcirculation.
    13. (1999). Correlation—mathematics and worked examples. In: Altman DG (ed) Practical statistics for medical research,
    14. (2002). Das ‘‘spatial shift alignment (SSA)’’—eine neue Methode zur Bestimmung der Blutflussgeschwindigkeit in de Video-Kapillarmikroskopie.
    15. (2004). Direct observation of the human cerebral microcirculation during aneurysm surgery reveals increased arteriolar contractility.
    16. (2006). Early microcirculatory perfusion derangements in patients with severe sepsis and septic shock: relationship to hemodynamics, oxygen transport, and survival. Ann Emerg Med (in press) 670 Med Biol Eng Comput
    17. (2006). Effects of drotrecogin alfa activated on microcirculatory alterations in patients with severe sepsis.
    18. (2004). Flow visualization tools for image analysis of capillary networks.
    19. (1992). Global processing via the Hough Transform. In:
    20. (1995). Gray-scale Hough transform for thick line detection in gray-scale images.
    21. (2002). Histogram equalization. In: Russ JC (ed) The image processing handbook, 4th edn.
    22. (2007). How to evaluate the microcirculation? Report of a round table conference. Crit Care 11:R101
    23. (2001). In vivo monitoring of microvessels in skin flaps: introduction of a novel technique.
    24. (2002). Measurement of the distribution of red blood cell deformability using an automated rheoscope.
    25. (2002). Microvascular blood flow is altered in patients with sepsis.
    26. (2005). Motion as orientation in space-time images. In: Ja ¨hne B (ed) Digital image processing, 6th revised and extended edn.
    27. (2002). Off-line analysis of red blood cell velocity in renal arterioles.
    28. (2002). OPS imaging op human microcirculation: a short technical report.
    29. (1999). Orthogonal polarization spectral imaging: a new method for study of the microcirculation.
    30. (2002). Oudemans-Van straaten HM, Zandstra DF
    31. (2004). Persistent microcirculatory alterations are associated with organ failure and death in patients with septic shock.
    32. (2005). Quantifying bedside-derived imaging of microcirculatory abnormalities in septic patients: a prospective validation study.
    33. (2004). Ridge-based vessel segmentation in color images of the retina.
    34. (2006). The effects of dobutamine on microcirculatory alterations in patients with septic shock are independent of its systemic effects.
    35. (2005). The microcirculation is the motor of sepsis. Crit Car 9(suppl 4):S13-S19
    36. (2005). The structure tensor. In: Ja ¨hne B (ed) Digital image processing, 6th revised and extended edn.
    37. (2006). Thrombolysis in fulminant purpura: observations on changes in microcirculatory perfusion during successful treatment.
    38. (2001). Validation of an analysis software for intravital microscopy with OPS imaging applied to the hamster skinfold chamber.
    39. (2001). Validation of an analysis software for OPS-imaging used in humans.

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