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Overexpression of Betaig-h3 gene downregulates integrin α5β1 and suppresses tumorigenicity in radiation-induced tumorigenic human bronchial epithelial cells

By Y L Zhao, C Q Piao and T K Hei

Abstract

Interaction between cell and extracellular matrix plays a crucial role in tumour invasion and metastasis. Using an immortalised human bronchial epithelial (BEP2D) cell model, the study here shows that expression of Betaig-h3 gene, which encodes a secreted adhesion molecule induced by transforming growth factor-β, is markedly decreased in several independently generated, radiation-induced tumour cell lines (TL1–TL5) relative to parental BEP2D cells. Transfection of Betaig-h3 gene into tumour cells resulted in a significant reduction in tumour growth. While integrin receptor α5β1 was overexpressed in tumour cells, its expression was corrected to the level found in control BEP2D cells after Betaig-h3 transfection. These data suggest that Betaig-h3 gene is involved in tumour progression by regulating integrin receptor α5β1. The findings provide strong evidence that the Betaig-h3 gene has tumour suppressor function in human BEP2D cell model and suggest a potential target for interventional therapy

Topics: Genetics and Genomics
Publisher: Nature Publishing Group
OAI identifier: oai:pubmedcentral.nih.gov:2375424
Provided by: PubMed Central

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Citations

  1. (2002). Acad Sci USA 92(13): 6161–6165 G e n e t i c s a n d G e n o m i c s Betaig-h3 inhibits tumorigenicity of human bronchial epithelial cells
  2. (2000). Expression of integrin alpha5beta1 and MMPs associated with epithelioid morphology and malignancy of uveal melanoma.
  3. (2000). Fluorescence in situ hybridization confirmation of 5q deletions in patients with hematological malignancies. Cancer Genet Cytogenet 117(1): 45–49 Bron AJ
  4. (2000). Significance of integrin alpha5 gene expression as a prognostic factor in node-negative non-small cell lung cancer.