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The complex between SOS3 and SOS2 regulatory domain from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis

By María José Sánchez-Barrena, Sandra Moreno-Pérez, Iván Angulo, Martín Martínez-Ripoll and Armando Albert

Abstract

Recombinant SOS3 and SOS2 regulatory domain from A. thaliana have been coexpressed in E. coli, purified and crystallized by the hanging-drop vapour-diffusion method. An X-ray data set has been collected at 2.0 Å resolution

Topics: Crystallization Communications
Publisher: International Union of Crystallography
OAI identifier: oai:pubmedcentral.nih.gov:2335134
Provided by: PubMed Central
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