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The Use of Amnion-Derived Cellular Cytokine Solution to Improve Healing in Acute and Chronic Wound Models

By Michael G Franz, Wyatt G Payne, Liyu Xing, D. K Naidu, R. E Salas, Vivienne S Marshall, C. J Trumpower, Charlotte A Smith, David L Steed and M. C Robson


Objective: Growth factors demonstrate mixed results improving wound healing. Amnion-derived multipotent cells release physiologic levels of growth factors and tissue inhibitors of metalloproteinases. This solution was tested in models of acute and chronic wound healing. Methods: Acute model: Sprague-Dawley rats underwent laparotomy incisions. The midline fascia was primed with phosphate-buffered saline, unconditioned media, or amnion-derived cellular cytokine suspension prior to incision. Breaking strength of laparotomy wounds was tested with an Instron tensiometer. Incisional hernia formation was measured after 28 days. Chronic model: Chronic, infected granulating wounds were produced in rats by excising full thickness burn eschars inoculated with Escherica coli. Granulating wounds were treated with unconditioned media or amnion-derived cellular cytokine suspension. Treatments were applied either on day 0 and day 7 or day 0 and then every other day. Wounds were traced every 72 hours and biopsied for quantitative bacteriology. Results: Acute model: Priming with amnion-derived cellular cytokine suspension increased the breaking strength of laparotomy incisions in comparison with phosphate-buffered saline or unconditioned media (P < .05). Acute wound failure and incisional hernia formation was 100% in the phosphate-buffered saline and unconditioned media groups and 18% in the amnion-derived cellular cytokine suspension–treated group (P <.05). Chronic model: The rate of wound closure was accelerated in amnion-derived cellular cytokine suspension–treated chronic wounds (P < .05). Multidosing improved the effect. Conclusions: A physiologic solution of cytokines and tissue inhibitors of metalloproteinases improves healing in models of acute and chronic wounds. Such a cocktail can be produced from amnion-derived multipotent progenitor cells

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    1. (1992). A mathematical model for the analysis of experimental wound healing data. Wounds.
    2. Amnion-derived cellular cytokine solution (ACCS): a physiological combination of cytokines for wound healing.
    3. Amnion-derived multipotent progenitor cells increase gain of incisional breaking strength and decrease incidence and severity of acute wound failure.
    4. (1991). Animal models of wound contraction.
    5. Bacterial degradation of growth factors.
    6. Basic fibroblast growth factor in a carboxymethylcellulose vehicle reverses the bacterial retardation of wound contraction.
    7. Bone marrow cells differentiate into wound myofibroblasts and accelerate the healing of wounds with exposed bones when combined with an occlusive dressing.
    8. Changes in growth factor levels in human wound fluid.
    9. (1986). Classification and treatment of chronic nonhealing wounds: successful treatment with autologous platelet-derived wound healing factors (PDWHF). Ann Surg.
    10. Constitutive and induced cytokine production by human placenta and amniotic membrane at term.
    11. (2003). Cytokine manipulation of the wound. Clin Plast Surg.
    12. (1998). Cytokine secretion by human fetal membranes, decidua, and placenta at term. Hum Reprod.
    13. DubayDA,WangX,AdamsonBS,KuzonWMJr,DennisRG,FranzMG.Progressivefascialwoundfailure impairs subsequent abdominal wall repairs: a new animal model of incisional hernia formation.
    14. Early prediction of late incisional hernias.
    15. (2004). Effect of cytokine growth factors on the prevention of acute wound failure. Wound Repair Regen.
    16. (1995). Expression of messenger ribonucleic acid for epidermal growth factor (EGF), transforming growth factor-alpha (TGF alpha), and EGF receptor in human amnion cells: possible role of TGF alpha in prostaglandin E2 synthesis and cell proliferation.
    17. Fascial incisions heal faster than skin: a new model of abdominal wall repair.
    18. Granulocyte-macrophage colony stimulating factor reverses the inhibition of wound contraction caused by bacterial contamination.
    19. (1995). In vivo characterization of interleukin-4 as a potential wound healing agent. Wound Repair Regen.
    20. (2007). Incisional herniation induces decreased abdominal wall compliance via oblique muscle atrophy and fibrosis. Ann Surg.
    21. (1985). of Health, Public Health Service.Guide for the Care of Laboratory Animals.
    22. (2005). Potential of human bone marrow stromal cells to accelerate wound healing in vitro. Ann Plast Surg.
    23. (2002). Ratios of activated matrix metalloproteinase-9 to tissue inhibitor of matrix metalloproteinase-1 in wound fluids are inversely correlated with healing of pressure ulcers. Wound Repair Regen.
    24. (1999). SolerPM,WrightTE,SmithPD,etal.Invivocharacterizationofkeratinocytegrowthfactor-2asapotential wound healing agent. Wound Repair Regen.
    25. (1974). The efficacy of systemic antibiotics in the treatment of granulating wounds. J Surg Res.
    26. (2004). The prevention of incisional hernia formation using a delayed-release polymer of basic fibroblast growth factor. Ann Surg.
    27. Transforming growth factor –beta(2) lowers the incidence of incisional hernias.
    28. Two peptides related to platelet-derived growth factor are present in human wound fluid.
    29. Variations on a theme. In: Heggers

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