Reduced Ca2-calmodulindependent protein kinase activity and expression in LV myocardium of dogs with heart failure

Abstract

dent protein kinase activity and expression in LV myocar-dium of dogs with heart failure. Am J Physiol Heart Circ Physiol 284: H876–H883, 2003. First published November 7, 2002; 10.1152/ajpheart.00266.2002. — Studies on the status of multifunctional Ca2-calmodulin (CaM)-dependent pro-tein kinase-II (CaMKII) in failing hearts are limited and controversial. The study was performed in the left ventricu-lar (LV) myocardium of six dogs with heart failure (HF) (LV ejection fraction, 23 2%) and six normal (NL) dogs. In the LV homogenate, CaMKII activity and its protein level were determined by using the CaMKII peptide and antibody, re-spectively. Furthermore, the protein level of CaM and phos-phorylated phospholamban (PLB) at threonine-17 (PLB-Thr17) and serine-16 (PLB-Ser16) were also determined in the LV homogenate using a specific antibody. In addition, th