QARIP: a web server for quantitative proteomic analysis of regulated intramembrane proteolysis

Abstract

Regulated intramembrane proteolysis (RIP) is a critical mechanism for intercellular communication and regulates the function of membrane proteins through sequential proteolysis. RIP typically starts with ectodomain shedding of membrane proteins by extracellular membrane-bound proteases followed by intramembrane proteolysis of the resulting membrane-tethered fragment. However, for the majority of RIP proteases the corresponding sub-strates and thus, their functions, remain unknown. Proteome-wide identification of RIP protease sub-strates is possible by mass spectrometry-based quantitative comparison of RIP substrates or their cleavage products between different biologica