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zeta-COP, a subunit of coatomer, is required for COP-coated vesicle assembly

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Abstract

cDNA encoding the 20-kD subunit of coatomer, zeta-COP, predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to zeta-COP blocks the binding of coatomer to Golgi membranes and prevents the assembly of COP-coated vesicles on Golgi cisternae. Unlike other coatomer subunits (beta-, beta'-, gamma-, and epsilon- COP), zeta-COP exists in both coatomer bound and free pools

Topics: Articles
Publisher: The Rockefeller University Press
OAI identifier: oai:pubmedcentral.nih.gov:2290901
Provided by: PubMed Central
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    Citations

    1. (1993). 3-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo.
    2. (1991). A coat subunit of Golgi-derived non-clathrincoated vesicles with homology to the clathrin-coated vesicle coat protein B-adaptin.
    3. (1986). A microtubule-binding protein associated with membranes of the Golgi apparatus.
    4. (1986). A new type of coated vesicular carrier that appears not to contain clathrin: its possible role in protein transport within the Golgi stack.
    5. (1992). ADPRibosylation factor, a small GTP-binding protein, is required for binding of the coatomer protein/3-COP to Golgi membranes.
    6. (1987). An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res.
    7. (1991). AP17 and AP19, the mammalian small chains of the clathrinassociated protein complexes show homology to YaplTp, their putative homolog in yeast.
    8. (1985). Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
    9. (1991). Binding of ARF and fl-COP to Golgi membranes: Possible regulation by a trimeric G protein. Science (Wash.)
    10. (1993). Binding of coatomer to Golgi membranes requires ADP-ribosylation factor.
    11. (1991). Brefeldin A, a drug that blocks secretion, prevents the assembly of non-clathrin-coated buds on Golgi cisternae.
    12. (1993). Budding from Golgi membranes requires the coatomer complex of non-clathrin coat proteins.
    13. (1990). Clathrin and associated assembly and disassembly proteins.
    14. (1970). Cleavage of structural protein during the assembly of the head of bacteriophage T4.
    15. (1991). Coatomer': a cytosolic protein complex containing subunits of non-clathrin-coated Golgi transport vesicles.
    16. (1991). COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to/~-adaptin.
    17. (1992). COP, a coat subunit of non-clathrincoated vesicles with homology to Sec21p.
    18. (1989). Dissection of a single round of vesicular transport: sequential intermediates for intercisternal movement in the Golgi stack.
    19. (1989). Examination of automated polypeptide sequencing using standard phenyl isothiocyanate reagent and subpicomole high-performance liquid chromatographic analysis.
    20. (1993). fl'-COP, a novel subunit of coatomer.
    21. (1988). Immunoaltinity purification of antibody. Antibodies:
    22. (1990). Internal sequence analysis of proteins separated on polyacrylamide gels at the sub microgram level: Improved methods, applications and gene cloning strategies.
    23. (1975). Intracellular aspects of the process of protein synthesis.
    24. (1989). Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack.
    25. (1988). Rapid production of full-length cDNAs from rare transcripts; amplification using a single genespecific oligonucleotide primer.
    26. (1984). Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine.
    27. (1992). SEC21 is a gene required for ER to Golgi protein transport that encodes a subunit of a yeast coatomer.
    28. (1982). Specimen preparation for electron microscopy using low temperature embedding resins..1".
    29. (1993). The binding ofAP-I clathrin adaptor particles to Golgi membranes requires ADP-ribosylation factor, a small Kuge et al. The Coat Protein ~-COP 1733 GTP-binding protein.
    30. (1978). Ultrastructural localization of intracellular antigens by the use of protein A-gold complex.

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