Location of Repository

Synthesis and Bioconjugation of Gold Nanoparticles as Potential Molecular Probes for Light-Based Imaging Techniques

By Raja Gopal Rayavarapu, Wilma Petersen, Constantin Ungureanu, Janine N. Post, Ton G. van Leeuwen and Srirang Manohar


We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the “optical imaging window.” The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed

Topics: Research Article
Publisher: Hindawi Publishing Corporation
OAI identifier: oai:pubmedcentral.nih.gov:2266791
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles



    1. (2000). Angiogenesis in cancer and other diseases,”
    2. (2005). Anisotropic metal nanoparticles: synthesis, assembly, and optical applications,”
    3. (2004). Bioconjugated gold nanoparticles as a molecular based contrast agent: implications for imaging of deep tumors using optoacoustic tomography,” Molecular Imaging and Biology,v o l .6 ,n o .5 ,p p .
    4. (2006). Cancer cell imagingandphotothermaltherapyinthenear-infraredregion by using gold nanorods,”
    5. (2002). Contrast agents for optical imaging,” in Contrast AgentsII:Optical,Ultrasound,X-RayandRadiopharmaceutical Imaging,
    6. (2001). D.A.Boas,D.H.Brooks,E.L.Miller,etal.,“Imagingthebody with diffuse optical tomography,”
    7. (2005). Deep tissue two-photon microscopy,”
    8. (1994). Discrete-dipole approximation for scattering calculations,”
    9. (2005). Duyne, “Biological applications of localised surface plasmonic phenomenae,”
    10. (2001). Evidence for bilayer assembly of cationic surfactants on the surface of gold nanorods,”
    11. (2005). Gold Nanorods: Synthesis,
    12. (2005). Mechanism of silver(I)-assisted growth of gold nanorods and bipyramids,”
    13. (1989). Methods for synthesis of colloidal gold,”
    14. (2003). n t e s ,J .R i p o l l ,Y .C h e n ,S .N i o k a ,A .G .Y o d h ,a n dB . Chance, “In vivo continuous-wave optical breast imaging enhanced with indocyanine green,”
    15. (2000). Non-invasive in vivo characterization of breast tumours using photon migration spectroscopy,”
    16. (2003). Optical coherence tomography for ultrahigh resolution in vivo imaging,”
    17. (2005). Optical sectioning microscopy,”
    18. (2005). Optically tunable nanoparticle contrast agents for early cancer detection: model-based analysis of gold nanoshells,”
    19. (2004). Optoacoustic imagingofgoldnanoparticles targeted to breast cancer cells,” in P h o t o n sP l u sU l tr a s o u n d :I m a g -ingandSensing,vol.5320ofProceedingsoftheSPIE,pp.21–28,
    20. (2006). Photoacoustic imaging in biomedicine,”
    21. (2004). Photoacoustic mammography laboratory prototype: imaging of breast tissue phantoms,”
    22. (2001). Photoacoustic pointsource,”PhysicalReviewLetters,vol.86,no.16,pp.3550– 3553,
    23. (2003). Preparation and growth mechanism of gold nanorods (NRs) using seed-mediated growth method,”
    24. (1996). Preparation of colloidal-gold-labeled proteins,”
    25. (2006). Quantitation of metal content in the silver-assisted growth of gold nanorods,”
    26. (1996). Quantitative opticalspectroscopyfortissuediagnosis,”Annual Review of
    27. (2003). Real-time vital optical imaging of precancer using anti-epidermal growth factor receptor antibodies conjugated to gold nanoparticles,”
    28. (1987). S l a m o n ,G .M .C l a r k
    29. (2005). Scanning time-domain optical mammography: detection and characterization of breast tumors
    30. (2001). Seed-mediated growth approach for shape-controlled synthesis of spheroidal and rod-like gold nanoparticles using a surfactant template,”
    31. (2004). Seed-mediated synthesis of gold nanorods: role of the size and nature of the seed,”
    32. (2004). Seeded high yield synthesis of short au nanorods in aqueous solution,”
    33. (2005). The Twente Photoacoustic Mammoscope: system overview and performance,”
    34. User Guide for the Discrete Dipole Approximation Code DDSCAT.6.1,” http://arxiv.org/ abs/astro-ph/0409262.

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.