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Purification, crystallization and preliminary X-ray crystallographic analysis of the outer membrane lipoprotein NlpE from Escherichia coli

By Yu Hirano, Md. Motarab Hossain, Kazuki Takeda, Hajime Tokuda and Kunio Miki

Abstract

A water-soluble mutant of the outer membrane lipoprotein NlpE has been overexpressed, purified and crystallized. Diffraction data from two crystal forms obtained under two different conditions were collected to 2.8 and 3.0 Å resolution and processed in space groups P43212 and C2, respectively

Topics: Crystallization Communications
Publisher: International Union of Crystallography
OAI identifier: oai:pubmedcentral.nih.gov:2225377
Provided by: PubMed Central
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