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Chemical and Morphological Studies of Bacterial Spore Formation : I. The Formation of Spores in Bacillus cereus

By I. Elizabeth Young and Philip C. Fitz-James


Experimental conditions were developed whereby a culture of Bacillus cereus formed spores with reasonable synchrony following a growth cycle of some 8 hours. The cytology of this metamorphosis was studied by dark phase contrast, bright-field microscopy and electron microscopy of thin sections. Particular attention has been paid to the changes in chromatin patterns and these have been correlated with quantitative chemical estimations of the nucleic acids. The cell commencing sporulation contains two compact chromatin bodies and twice the spore amount of deoxyribonucleic acid. Following fusion of the two chromatin bodies, one-half of this chromatin becomes located at a cell end. A transverse septum growing inwards from, and remaining attached to, the inner surface of the cell wall separates this end-piece of chromatin and some associated cytoplasm from the rest of the cell to form the primordial spore. Although the synthesis of deoxyribonucleic acid ceases during the segregation process, it recommences in this organism and continues at a linear rate as the spore develops. Tracer studies with radioactive phosphorus indicated that this further synthesis is confined to the non-spore portion of the sporangium. Although the net synthesis of ribonucleic acid ceased prior to the onset of sporogenesis, some evidence of a turnover of this fraction during the sporulation process was found

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    1. A comparison of species and varieties of the genus Bacillus based on the structure and nucleic acid content of their spores,
    2. (1945). A method for the determination of desoxyribonucleic acid, ribonucleic acid and phospho-proteins in animal tissues,
    3. A method for the determination of tracer phosphate in biological material,
    4. (1952). A method for varying the average number of nuclei in the conidia of Neurospora crassa,
    5. (1940). A simple culture medium for general use without meat extract or peptone,
    6. (1948). A tissue disintegrator,
    7. (1940). Bacterial spores as antigens,
    8. (1945). Changes in the nuclear structure of bacteria particularly during spore formation,
    9. (1958). Chemical and morphological changes during sporulation in variants of Bacillus cereus,
    10. (1959). Chemical and morphological studies of bacterial spore formation. II. Spore and parasporal protein formation in Bacillus cereus
    11. (1959). Chemical and morphological studies of bacterial spore formation. II1. The effects of 8-azaguanine on spore and parasporal protein formation in Bacillus cereus
    12. Chemical and morphological studies of bacterial spore formation. IV. The development of spore refractility, 1959, to be published. 482 BACTERIAL SPORE FORMATION.
    13. (1933). Cytological changes during the formation of the endospore in Bacillus megaterium,
    14. (1950). Demonstration of the chromatinic bodies of Escherichia coli and Proteus vulgarls with the aid of the phase contrast microscope,
    15. (1941). Die organismen des Sauerteigs und ihre Bedeutung fur die Brotgahrung,
    16. (1933). Eine cytologische Untersuchung ueber das Chromatin und den Entwicklungszklus der Bakterien,
    17. (1953). Electron-microscopy of ultra-thin sections of bacteria. I. Cellular division in Bacillus cereus,
    18. (1956). Electron-microscopy of ultra-thin sections of bacteria. II. Sporulation of Bacillus megaterium and Bacillus
    19. (1959). Fine structure of Bacillus subtilis.
    20. (1951). Le determinisme de la sporulation de Bacillus megaterium. I. L'effet de l'epuisement de l'allment carbone en milieu synthetique,
    21. (1948). Nuclear changes in Bacillus anthracis and their relation to variants,
    22. (1956). Nuclear division as observed in live bacteria by a new technique,
    23. (1950). Observations on the cytology of bacteria,
    24. (1953). Observations on the nucleus of resting and germinating spores of Bacillus megaterium,
    25. (1951). Observations on the structure of spores,
    26. (1957). Observations with the electron microscope on the organization of the cortex of resting and germinating spores of B.
    27. (1945). Phosphorus compounds in animal tissues,
    28. Some cytochemical contributions to genetic chemistry, in
    29. (1958). Studies on the fine structure of microorganisms. II. Electron microscopic studies on sporulation of Clostridium sporogenes,
    30. (1956). The chromatin bodies of bacteria,
    31. (1956). The chromatin bodies of bacteria~ Bact. Rev.,
    32. (1941). The cytology of bacteria,
    33. (1956). The effects of the ionic environment on the chromatin structures of bacteria,
    34. (1952). The nuclear cytology of sporulation in Bacillus megaterium,
    35. (1959). The pattern of synthesis of deoxyribonucleic acid in B. cereus growing synchronously out of spores, Nature,
    36. (1955). The phosphorus fractions of Bacillus cereus and Bacillus megaterium. I. A comparison of spores and vegetative cells,
    37. (1955). The phosphorus fractions of Bacillus cereus and Bacillus megaterium. II. A correlation of the chemical with the cytological changes occurring during spore germination,
    38. (1941). Ueber Geissein, Reservestoffe, Kerne und Sporenbildung der Bacterien,
    39. Ueber Morphologie und Entwichlungsgeschichte einigen endosporer

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