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Diminished interferon-gamma (IFN-γ) production by bacterial antigen-specific T cells in atopic patients

By N SHIMOJO, Y KOHNO, T KATSUKI, A HOSHIOKA, K HONMA, K SAITO and H NIIMI

Abstract

In this study, we established and studied cytokine production of T cell lines (TCL) specific to either a purified protein derivative of Mycobacterium tuberculosis (PPD) or Dermatophagoides farinae (Df) from atopic patients and non-atopic healthy subjects. IFN-γ was detected in the culture supernatants of all of 36 PPD-specific TCL established from healthy controls, whereas only 24 of 38 PPD-specific TCL from patients produced IFN-γ. Furthermore, the amounts of IFN-γ produced by PPD-specific TCL from patients were significantly lower than those from healthy controls. No IL-4 was detected in any PPD-specific TCL from either healthy controls or atopic patients. The amounts of IL-4 production from Df-specific TCL from atopic patients were much higher than from healthy controls, while few TCL produced IFN-γ. These results suggest that the skewing to the Th2-type T cell response in atopic patients is a response not only to allergens, but also to bacterial antigens, compared with non-atopic subjects. Activation of PPD-specific TCL from patients with calcium ionophore A23187 plus phorbol myristate acetate resulted in much higher IFN-γ production than in TCL established from healthy controls, indicating that the low production of IFN-γ by PPD-specific T cells from atopic patients is not due to an intrinsic T cell defect but to some regulatory mechanisms

Topics: Original Articles
Publisher: Blackwell Science Inc
OAI identifier: oai:pubmedcentral.nih.gov:2200563
Provided by: PubMed Central
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