A rapid gas-liquid chromatographic determination of serum levels of phenobarbital and diphenylhydantoin

Abstract

This report describes a simple gas-liquid chromatographic method for the simultaneous determination of phenobarbital and diphenylhydantoin in serum or plasma. The procedure involves a simple extraction with an isobutylmethyl ketone/n-hexane mixture, on-column ethylation with a methanolic solution of tetraethylammonium hydroxide, and analysis on a flame ionisation Chromatograph. This method offers advantages of accuracy, sensitivity and rapidity. No interference from other constituents of blood was found. This reliable technique is well adapted to the routine monitoring of epileptic patients. © 1975.SCOPUS: ar.jinfo:eu-repo/semantics/publishe