Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase

Veedu, R.N.; Vester, B.; Wengel, J.

oai:researchrepository.murdoch.edu.au:27434

Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase

Authors: R.N. Veedu
B. Vester
J. Wengel
Publication date: 1 January 2009
Publisher: 'Royal Society of Chemistry (RSC)'

Abstract

Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions. Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex

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