Skip to main content
Article thumbnail
Location of Repository

Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase

By R.N. Veedu, B. Vester and J. Wengel

Abstract

Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions. Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex

Publisher: Royal Society of Chemistry
Year: 2009
OAI identifier: oai:researchrepository.murdoch.edu.au:27434
Provided by: Research Repository
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://researchrepository.murd... (external link)
  • http://researchrepository.murd... (external link)
  • http://researchrepository.murd... (external link)
  • http://researchrepository.murd... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.