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Rapid separation of seed gliadins by reversed-phase ultra performance liquid chromatography (RP-UPLC) and its application in wheat cultivar and germplasm identification

By C. Han, X. Lu, Z. Yu, X. Li, W. Ma and Y. Yan

Abstract

To separate gliadin from wheat flour, a novel and stability-indicating reversed-phase ultra performance liquid chromatography (RP-UPLC) method is established and optimized. A comparative analysis of routine capillary electrophoresis (CE), reversed-phase high-performance liquid chromatography (RP-HPLC), and RP-UPLC was performed and the results showed that the resolution and efficiency of RP-UPLC were significantly higher than those of CE and RP-HPLC. Characteristic RP-UPLC patterns of different bread wheat variety and related species were readily identified. These results demonstrated that our RP-UPLC procedure resulted in significant improvements in sensitivity, speed, and resolution, and thus is highly useful in wheat cultivar and germplasm identification.\ud The novel UP-UPLC method showing high resolution and efficiency in the separation of seed gliadins might be applied to wheat variety and germplasm identification

Publisher: Taylor & Francis
Year: 2015
OAI identifier: oai:researchrepository.murdoch.edu.au:27055
Provided by: Research Repository
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