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DNA Sequences of Parachlamydia-like organisms in CSF of Patients with Neurological Disorders

By C. Contini, S. Seraceni, M. Castellazzi, C. Tamborino, E. Granieri and E. Fainardi

Abstract

Previously our findings have suggested a possible association between C. pneumoniae and Chlamydia-like organism brain infections as a cofactor in Multiple Sclerosis (MS) development (Contini et al, 2008). In particular, the DNA sequences found exhibited 83.8% of similarity with Neochlamydia sp. LTUNC08556 16s rRNA and 67.9% with Endosymbiont of Acanthamoeba sp. UWC22 16s rRNA. On the basis of these results, and considering that other Chlamydia-related bacteria, including Simkania negevensis, Waddlia chondrophila and the recently discovered bacterium Protochlamydia naegleriophila might also cause human infections, we wanted to deepen and study other patients with different neurological disorders other than MS. A total of 105 CSF samples were collected from MS patients (n. 20), other inflammatory neurological diseases (n. 49, OIND) and non-inflammatory neurological diseases (n. 36, NIND). DNA was extracted from fresh CSF samples and PCR was carried out with primers targeting the 16S rRNA gene according to Greub (2006). We detected 7 (6,6%) positive specimens: 2 MS (10%), 2 OIND (4,1%) with polyneuritis and 3 NIND (8.3%) with chronic ischemia, parestesia and cerebral ischemic vasculopathy. In all cases the positive amplified PCR products corresponded to 261 bp. These were purified, sequenced and compared (BLAST program, http://www.ncbi. nlm.nih.gov/BLAST) with other gene sequences to assess the homology or percentage of identity. In MS and OIND patients, BLAST anaysis revealed Protochlamydia naegleriophila strain KNic 16S ribosomal RNA gene and Endosymbiont of Acanthamoeba sp. UWE1 16S ribosomal RNA gene (rates of homology 92%-93% and 99%-79%, respectively). In NIND patients, Endosymbiont of Acanthamoeba sp, Protochlamydia naegleriophila strain KNic 16S and Parachlamydia-related symbiont UWE25 (rates of homology 91%, 83% 81%, respectively) were found. The exact role of Parachlamydiaceae in human diseases and MS is not well defined. So far, Protochlamydia naegleriophila, a new uncultivable intracellular bacterium, was recently detected as potential agent of community-acquired pneumonia but its role is yet under investigation. The detection of this pathogen in MS as well as in neurological disorders is a new finding which may have potential clinical implications but needs to be further explored

Topics: Chlamydia-like organism, brain infections, Multiple Sclerosis (MS), DNA sequences, BLAST program, Chlamydophila pneumoniae
Publisher: -ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 24-28 OVAL RD, LONDON, ENGLAND, NW1 7DX -Elsevier Science Limited:Oxford Fulfillment Center, PO Box 800, Kidlington Oxford OX5 1DX United Kingdom:011 44 1865 843000, 011 44 1865 843699, EMAIL: asianfo@elsevier.com, tcb@elsevier.co.UK, INTERNET: http://www.elsevier.com, http://www.elsevier.com/locate/shpsa/, Fax: 011 44 1865 843010
Year: 2010
OAI identifier: oai:iris.unife.it:11392/1682720
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