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Human mesenchymal stem cells seeded on extracellular matrix scaffold: Viability and osteogenic potential.

By Penolazzi L., Mazzitelli S., Vecchiatini R., Torreggiani E., Lambertini E., Johnson S., Badylak S.F., Piva R. and Nastruzzi C.


The development and the optimization of novel culture systems of mesenchymal osteoprogenitors are some of the most important challenges in the field of bone tissue engineering. A new combination between cells and ECM-scaffold, containing extracellular matrix (ECM) has here been analyzed. As source for osteoprogenitors, mesenchymal stem cells obtained from human umbilical cord Wharton's Jelly (hWJMSCs), were used. As ECM-scaffold, a powder form of isolated and purified porcine urinary bladder matrix (pUBM), was employed. The goals of the current work were: (1) the characterization of the in vitro hWJMSCs behaviour, in terms of viability, proliferation and adhesion to ECM-scaffold; (2) the effectiveness of ECM-scaffold to induce/modulate the osteoblastic differentiation and (3) the proposal for a possible application of cells/ECM-scaffold construct to the field of cell/tissue engineering.\ud \ud In this respect, the properties of the pUBM-scaffold in promoting and guiding the in vitro adhesion, proliferation and three-dimensional colonization of hWJMSCs, without altering viability and morphological characteristics of the cells, are here described. Finally, we have also demonstrated that pUBM-scaffolds positively affect the expression of typical osteoblastic markers in hWJMSCs

Topics: urinary bladder matrix, human mesenchymal stem cells, osteoblast differentiation
Year: 2012
DOI identifier: 10.1002/jcp.22983
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