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An assessment of the antibacterial activity in larval excretion/secretion of four species of insects recorded in association with corpses, using Lucilia sericata Meigen as the marker species

By K. M. Barnes, D. E. Gennard and R. A. Dixon

Abstract

The relative antibacterial activities of excretion/secretion (ES) from two carrion-feeding\ud insects, Calliphora vicina Robineau-Desvoidy and Dermestes maculatus\ud DeGeer, and a detritivore, Tenebrio molitor Linnaeus, were compared to that of\ud Lucilia sericata Meigen, a species with ES of known antibacterial capacity, in order\ud to explore the antimicrobial potential of other carrion and detritivore species.\ud Viable counts were used to assess time-kill of ES against five bacterial species,\ud Staphylococcus aureus, Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa and\ud Proteus mirabilis. Antibacterial activity was recorded in all four insect species\ud although T. molitor and D. maculatus were the most effective in controlling growth\ud of P. mirabilis. The blowflies were more effective in controlling a wider range of\ud both Gram-positive and Gram-negative bacteria. The larval ES from all species was\ud shown to reduce bacterial growth rate although differences in antibacterial\ud spectrum were noted and the degree of potency varied between the four species.\ud These differences may be explained ecologically by the different colonisation times\ud of each insect species on the corpse. Overall, this study demonstrates that research\ud into other carrion-feeding insect species has potential to provide an increased\ud source of antimicrobial chemicals to broaden the range of bacterial species beyond\ud that currently controlled using L. sericata

Topics: D320 Animal Health, F410 Forensic Science, C340 Entomology
Publisher: Cambridge University Press
Year: 2010
DOI identifier: 10.1017/S000748530999071X
OAI identifier: oai:eprints.lincoln.ac.uk:2515

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