This paper compares two manual measurement\ud techniques for measuring retinal vessel segment widths: the\ud kick-points technique and the edge marking technique. An image\ud set of 164 clear, high-resolution segments was used. The\ud kick-points approach uses kick points marked by observers\ud along interpolated cross-sectional intensity profile graphs; the\ud edge marking method allows observers to nominate the edges on\ud a zoomed-up image, and interpolates edge positions. The edgemarking\ud method provides more precise measurements than the\ud kick-points method, but these are subject to more inter-observer\ud variability; we speculate that this result is due to differing observer\ud perceptions of the edge location
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