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Combining calcium imaging with other optical techniques. : Ca 2+ Imaging and Other Optical Techniques

By Marco Canepari, Dejan Zecevic, Kaspar Vogt, David Ogden and Michel De Waard

Abstract

International audienceCa(2+) imaging is a commonly used approach for measuring Ca(2+) signals at high spatial resolution. The method is often combined with electrode recordings to correlate electrical and chemical signals or to investigate Ca(2+) signals following an electrical stimulation. To obtain information on electrical activity at the same spatial resolution, Ca(2+) imaging must be combined with membrane potential imaging. Similarly, stimulation of subcellular compartments requires photostimulation. Thus, combining Ca(2+) imaging with an additional optical technique facilitates the study of a number of physiological questions. The aim of this article is to introduce some basic principles regarding the combination of Ca(2+) imaging with other optical techniques. We discuss the design of the optics, the design of experimental protocols, the optical characteristics of Ca(2+) indicators used in combination with an optical probe, and the affinity of the Ca(2+) indicator in relation to the type of measurement. This information will enable the reader to devise an optimal strategy for combined optical experiments

Topics: [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Publisher: Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press
Year: 2013
DOI identifier: 10.1101/pdb.top066167
OAI identifier: oai:HAL:inserm-00951425v1
Provided by: Hal-Diderot

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