Distinctive autofluorescence of urine samples from individuals with bacteriuria compared with normals

Abstract

Background A variety of fluorophores are present in normal human urine. Alteration in the autofluorescence of urine could result from physiological or pathological changes. Method This study investigates the differences in the autofluorescence of 45 normal urine samples from 25 individuals with bacteriuria. Results Excitation at 290 nm showed good discrimination between these 2 groups. Principal Component Analysis (PCA) of the data revealed statistically significant differences between the fluorescence spectra for samples with bacteriuria as compared to the control group. Conclusion The findings indicate the potential of the fluorescence spectrum of urine to be developed as a simple and rapid diagnostic tool.3 page(s