Location of Repository

Quantitative analysis of chloroplast protein targeting

By Michael Kok Keng Li

Abstract

This thesis presents the first use of the Partition of Unity Method in quantifying\ud the spatio-temporal dynamics of a fluorescent protein targeted to\ud the chloroplast twin-arginine translocation pathway.\ud The fluorescence loss in photobleaching technique is applied in a modified\ud fashion to the measurement of substrate mobilities in the chloroplast\ud stroma. Our in vivo results address the two suggested protein targeting\ud mechanisms of membrane-binding before lateral movement to the translocon\ud and direct binding to the translocon.\ud A high performance computing C/C++ implementation of the Partition\ud of Unity Method is used to perform simulations of fluoresence loss\ud in photobleaching and allow a compelling comparison to photobleaching\ud data series. The implementation is both mesh-free and particle-less

Topics: QK
OAI identifier: oai:wrap.warwick.ac.uk:3917

Suggested articles

Preview

Citations

  1. 43Chapter 1. Introduction Stiness matrix The stiness matrix is used to calculate R jruhj2
  2. 66Chapter
  3. (2003). A particle-partition of unity method – Griebel and Schweitzer Part V: boundary conditions. doi
  4. as the loss in fluorescence is account
  5. C C C C C A 1=2 is a norm on Wm;2( ). H1( ) is defined to be the completion of the space C1( ) with respect to the
  6. C C C C C C C C C C C C C C C A Namingbasedonphysicalinterpretation Thegeneralformofadamped wave equation @2u @t2 + @u @t r (ru) = 0 gives rise to a “matrix dierential equation” (Zienkiewicz et al., 2006, p. 564) M¨ q + C˙ q + Kq + f
  7. contrast with a symmetric approximation in space. 192Bibliography RobertA.Adams.SobolevSpaces.,volume65ofPureandappliedmathematics; a series of monographs and textbooks. Academic Press, 1975.
  8. Disce Prodesse “Learn to
  9. (2005). Diusion on the endoplasmic Simulations of (an)isotropic reticulum membrane and FRAP diusion on curved biological (kernel-based particle method). surfaces.
  10. EMBO J, 18(11):2982–2990, 1999. doi:10.1093/emboj/18.11.2982. 6 Carole Dabney-Smith, Hiroki Mori, and Kenneth Cline. Oligomers of Tha4 organize at the thylakoid Tat translocase during protein transport.
  11. Essential cytoplasmic domains in
  12. Galerkin Method – Weak Formulation The Galerkin method approximates the solution of L(u) = f in doi
  13. Homann-Rohrer,TobiasA.Knoch,WaldemarWaldeck,andJörgLangowski. Analyzing intracellular binding and diusion with continuous fluorescence photobleaching. Biophys J, 84(5):3353–3363, 2003. 29, 36 M. B. Walker, L. M. Roy, E. Coleman, R. Voelker, and A. Barkan.
  14. Latin motto of the University of Warwick (established 1965). N.B. Abbreviation for Latin phrase
  15. (2003). likely finite dierence with Wachsmuth
  16. M A number M 2 N used to denote the number of test
  17. Mass matrix The mass matrix is used to calculate R (uh)2 dx and takes the form shown below (Strang
  18. Name Example Dirichlet or essential b.c. u = f Neumann or natural b.c. @u @n = 0 Robin or generalized Neumann b.c. au + b@u @n = g Mixed combinations of the above The
  19. Point in polygon test The point in polygon test we use is the HackerShimrat Algorithm 112 (Hacker, 1962). doi
  20. Rayleigh-Ritz Method
  21. Shepard’sMethod
  22. Svedberg unit (S) Non-SIunitnamedforSwedishchemistTheodorSvedberg (1884-1971). One Svedberg is 1013 seconds. (see
  23. TatB and TatC form a functional and
  24. that may be called the “finite element equation” (Strang and Fix, 1973, p. 32), or “system equations” (Zienkiewicz et al.,
  25. (2000). Year Title Author(s)

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.