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Quantitative analysis of chloroplast protein targeting

By Michael Kok Keng Li


This thesis presents the first use of the Partition of Unity Method in quantifying\ud the spatio-temporal dynamics of a fluorescent protein targeted to\ud the chloroplast twin-arginine translocation pathway.\ud The fluorescence loss in photobleaching technique is applied in a modified\ud fashion to the measurement of substrate mobilities in the chloroplast\ud stroma. Our in vivo results address the two suggested protein targeting\ud mechanisms of membrane-binding before lateral movement to the translocon\ud and direct binding to the translocon.\ud A high performance computing C/C++ implementation of the Partition\ud of Unity Method is used to perform simulations of fluoresence loss\ud in photobleaching and allow a compelling comparison to photobleaching\ud data series. The implementation is both mesh-free and particle-less

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