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Mandibular appliance modulates condylar growth through integrins

By M. Rubia MARQUES, D. HAJJAR, K. Gomes FRANCHINI, A. Sigari MORISCOT and M. Fagundes SANTOS

Abstract

Functional orthopedic therapy corrects growth discrepancies between the maxilla and mandible, possibly through postural changes in the musculature and modulation of the mandibular condylar cartilage growth. Using Wistar rats, we tested the hypothesis that chondrocytes respond to forces generated by a mandibular propulsor appliance by changes in gene expression, and that integrins are important mediators in this response. Immunohistochemical analyses demonstrated that the use of the appliance for different periods of time modulated the expression of fibronectin, alpha 5 and alpha v integrin subunits, as well as cell proliferation in the cartilage. In vitro, cyclic distension of condylar cartilage-derived cells increased fibronectin mRNA, as well as Insulinlike Growth Factor-I and II mRNA and cell proliferation. A peptide containing the Arginine-Glycine-Asparagine sequence (RGD), the main cell-binding sequence in fibronectin, blocked almost all these effects, confirming that force itself modulates the growth of the rat condylar cartilage, and that RGD-binding integrins participate in mechanotransduction

Topics: cartilage, IGF, fibronectin, mechanical force, ARTICULAR-CARTILAGE EXPLANTS, FACTOR-I, ALPHA-5-BETA-1 INTEGRIN, FIBRONECTIN METABOLISM, CYCLIC COMPRESSION, HUMAN CHONDROCYTES, MATRIX PROTEINS, GENE-EXPRESSION, YOUNG-RATS, MECHANOTRANSDUCTION, Dentistry, Oral Surgery & Medicine
Publisher: INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R
Year: 2013
OAI identifier: oai:agregador.ibict.br.RI_UNICAMP:oai:unicamp.sibi.usp.br:SBURI/645
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