Different patterns of cellular infiltrate and cytokines distinguish clinical forms of paracoccidioidomycosis

Abstract

A paracoccidioidomicose (PCM), micose sistêmica causada pelo fungo Paracoccidioides brasiliensis, apresenta um largo espectro de manifestações clínicas e imunológicas, variando de formas benignas e localizadas a quadros graves e disseminados. A forma crônica ou adulta (FA) se caracteriza por lesões localizadas com acometimento da pele, mucosa oral (MO) e pulmões; enquanto a forma aguda ou juvenil (FJ) é mais grave e disseminada com acometimento do fígado, baço e linfonodos (LNs). Nós examinamos a resposta inflamatória/imunológica em lesões de pacientes com formas clínicas distintas da PCM. Foram analisados a expressão de citocinas e o fenótipo das células presentes nos infiltrados de biópsias de mucosa oral de 15 pacientes com a forma adulta e de linfonodos de 15 pacientes com a forma juvenil da PCM por imuno-histoquímica (IHQ) e qPCR. Anticorpos monoclonais ou policlonais foram utilizados para caracterizar o infiltrado celular (CD68, CD15, CD3, CD8, CD56 e FOXP3), citocinas...Observação: O resumo, na íntegra, poderá ser visualizado no texto completo da tese digital.Paracoccidioidomycosis (PCM), a systemic disease caused by the fungus P. brasiliensis, presents with a wide spectrum of clinical and immunological manifestations, varying from benign and localized forms to severe and disseminated forms. The chronic or adult form (AF) is characterized by localized lesions with involvement of the skin, oral mucosa (OM) and lungs. While the acute or juvenile form (JF) is more severe and presents disseminated widespread with the involvement of the liver, spleen and lymph nodes (LNs). In order to examine the inflammatory/immune response in lesions of patients with different clinical forms of PCM, we analyzed the expression of cytokines as well as the phenotype of the cells in the inflammatory infiltrate. Paraffin-embedded OM biopsies from 15 patients with the localized AF of PCM and LN from 15 patients with the JF of PCM were analyzed by immunohistochemistry (IHC) and qPCR. Monoclonal or polyclonal antibodies were used to characterize the cellular infiltrate (CD68, CD15, CD3, CD8, CD56, and FOXP3), cytokines...Note: The complete abstract is available with the full electronic document