Encapsulamento de Beijerinckia sp utilizando spray-drier

Abstract

A escolha adequada do material de parede é um dos fatores determin antes no grau de viabilidade e na manutenção da atividade fermentativa do inóculo encapsulado. Maltodextrina, glicose, amido modificado e goma arábica foram testadas como encapsulantes. Beijerínckíaencapsulada em maltodextrina, reidratada e inoculada em meio de fermentação previamente esterilizado, após um período de estocagem de dois meses, apresentou maior estabilidade da atividade fermentativa. A sobrevivencia e a atividade fermentativa das células de Beijerínckía encapsulada em maltodextrina foram tes tadas sob várias condições do spray-drier e durante posterior período de estocagem. A viabilidade bacteriana para todas condições testadas apresentou declinio pórem quando se utilizou menores temperatura de saída no spray -drier e teor de sólidos na suspen são obteve-se maior grau de sobrevivência. Para verificar a influência da encapsulação em maltodextrina sobre a produção de biopolímero comparou -se a atividade fermentativa das células de Beijerínckía encapsulada e não encapsulada. A partir das característ icas reológicas e químicas dos biopolímeros produzidos e da cinética fermentativa dos inóculos concluiuise que encapsulaçao de Beijerínckía influe no crescimento celular e na produção de biopolímeroAn adequate choice of the wall (coating) material is one of the factors, which will determine the degree of cell survival and the maintenance of fermentative activity in the encapsulated inoculum. Malt dextrin, dehydrated glucose syrups, modified starch and acacia (gum Arabic) were used as wall ma terials. The results showed that spray dried Beijerinckia encapsulated in malt dextrin, stored for two months and inoculated into sterile must after re -hydration, presented the greatest stability related to fermentative activity and percent viability durin 9 spray drying and storage period. The survival and fermentative activity of Beijerinckia sp cultures encapsulated in malt dextrin by spray drying were determined under various conditions of spray drying and during the subsequent storage period of the products. The survival of Beijerinckia sp was greater with lower outlet air temperatures and with lower solids contents in the feed suspension during spray drying. Increasing either the spray dryer outlet air temperature or the feed solids content resulted in a decrease in the moisture content of the spray dried powders. A gradual decrease in viability was observed during storage for all treatments, although there was a relatively greater retention of fermenting activity in the inoculae obtained with lower outlet air temperatures and lower solids contents in the feed suspension. Was to examine the effects of encapsulation in malt dextrin on the production of biopolymer, comparing encapsulated and non-encapsulated Beijerinckia cells. From the characterization of the biopolymers and study of the fermentation kinetics, it was shown that the encapsulation of Beijerinckia mainly influenced cell growth and biopolymer productio

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