Skip to main content
Article thumbnail
Location of Repository

Lower prevalence of human immunodeficiency virus type 1 Brazilian subtype B found in northeastern Brazil with slower progression to AIDS.

By Adriano Fernando da Silva Araujo, Carlos Brites, Joana Paixão Monteiro Cunha, Luciane Amorim Santos, Bernardo Galvão Castro Filho and Luiz Carlos Júnior Alcantara


Besides being extremely useful in measuring the level of HIV-1 diversity and prevalence in populations, the molecular analysis of genomic sequences provides crucial surveillance support and aids in the development of new therapies and effective vaccines. The present study focused on gag and env DNA and amino acid sequences that were generated from samples taken from 61 infected patients in the City of Salvador, Bahia, located in northeastern Brazil. In order to determine selective pressure and predict coreceptor usage, Bioinformatics tools were employed in phylogeny reconstruction. Fifty-six (91.8%) viruses were classified as belonging to subtype B, three (4.9%) from F1, and two (3.3%) from BF1 recombinants. Based on the characterization of the V3 region, the subtype B strains were represented by eight (18.2%) Brazilian variants (B’-GWGR), 20 (46.5%) European/EUA B variants (GPGR), and 15 (34.9%) GXGX variants. The mean time elapsed since diagnosis was 13 years among subtype B’ and 9 years in subtype B. The mean dN/dS ratios from the GWGR, GPGR, and GXGX groups, when compared to an HXB2 reference, were 0.72, 0.77, and 0.67, respectively. Seventy-six percent of the viruses studied were predicted to use the CCR5 coreceptor for cell entry (R5 viruses), while 24% were predicted to use the CXCR4 or were classified as dual tropic viruses. The prevalence of subtypes B’ and recombinant B/F1 was shown to be lower than findings from previous studies performed both in Brazil (B’) and in Bahia (B/F1). The association between subtype B’ and a lengthy period of time since diagnosis can be correlated with a slower disease progression in infected patients, when compared with those infected with subtype B

Topics: Síndrome de Imunodeficiência Adquirida/epidemiologia, Síndrome de Imunodeficiência Adquirida/virologia, HIV-1/classificação, HIV-1/genética, Brasil/epidemiologia, Adulto, Análise por Conglomerados, Progressão da Doença, Feminino, Genótipo, HIV-1/isolamento & purificação, Humanos, Masculino, HIV-1/patogenicidade, Dados de Sequência Molecular, Filogenia, Prevalência, Receptores de HIV/fisiologia, Análise de Sequência de DNA, Homologia de Sequência, Produtos do Gene env do Vírus da Imunodeficiência Humana/genética
Publisher: Mary Ann Liebert, Inc
Year: 2010
OAI identifier:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • (external link)
  • Suggested articles

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.