Characterization of novel Leishmania infantum recombinant proteins encoded by genes from five families with distinct capacities for serodiagnosis of canine and human visceral leishmaniasis.
To expand the available panel of recombinant proteins that can be useful for identifying Leishmania -infected
dogs and for diagnosing human visceral leishmaniasis (VL), we selected recombinant antigens from L. infantum , cDNA,
and genomic libraries by using pools of serum samples from infected dogs and humans. The selected DNA fragments
encoded homologs of a cytoplasmic heat-shock protein 70, a kinesin, a polyubiquitin, and two novel hypothetical proteins.
Histidine-tagged recombinant proteins were produced after subcloning these DNA fragments and evaluated by using an
enzyme-linked immunosorbent assays with panels of canine and human serum samples. The enzyme-linked immunosorbent
assays with different recombinant proteins had different sensitivities (67.4–93.0% and 36.4–97.2%) and specificities
(76.1–100% and 90.4–97.3%) when tested with serum samples from Leishmania -infected dogs and human patients with
VL. Overall, no single recombinant antigen was sufficient to serodiagnosis all canine or human VL cases
Doenças do Cão/diagnóstico, Genes de Protozoários/genética, Leishmania infantum/genética, Leishmaniose Visceral/diagnóstico, Antígenos de Protozoários/genética, Animais, Clonagem Molecular, Doenças do Cão/parasitologia, Cães, Ensaio de Imunoadsorção Enzimática/métodos, Ensaio de Imunoadsorção Enzimática/veterinária, Proteínas de Choque Térmico HSP70/genética, Humanos, Cinesina/genética, Leishmaniose Visceral/parasitologia, Leishmaniose Visceral/veterinária, Poliubiquitina/genética, Proteínas Recombinantes/genética, Sensibilidade e Especificidade, Testes Sorológicos/métodos
Publisher: American Society of Tropical Medicine and Hygiene
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