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Characterization of novel Leishmania infantum recombinant proteins encoded by genes from five families with distinct capacities for serodiagnosis of canine and human visceral leishmaniasis.

By Geraldo Gileno de Sé Oliveira, Franklin Barbalho Magalhães, Márcia Cristina Aquino Teixeira, Andrea Mendes Pereira, Cristiane Garboggini Melo de Pinheiro, Lenita Ramires dos Santos, Marília Barabosa do Nascimento, Cheila Nataly Galindo Bedor, Alessandra Lima de Albuquerque, Washington Luis Conrado dos-Santos, Yara de Miranda Gomes, Edson Duarte Moreira Júnior, Maria Edileuza Felinto de Brito, Lain Carlos Pontes-de-Carvalho and Osvaldo Pompilio de Melo Neto


To expand the available panel of recombinant proteins that can be useful for identifying Leishmania -infected dogs and for diagnosing human visceral leishmaniasis (VL), we selected recombinant antigens from L. infantum , cDNA, and genomic libraries by using pools of serum samples from infected dogs and humans. The selected DNA fragments encoded homologs of a cytoplasmic heat-shock protein 70, a kinesin, a polyubiquitin, and two novel hypothetical proteins. Histidine-tagged recombinant proteins were produced after subcloning these DNA fragments and evaluated by using an enzyme-linked immunosorbent assays with panels of canine and human serum samples. The enzyme-linked immunosorbent assays with different recombinant proteins had different sensitivities (67.4–93.0% and 36.4–97.2%) and specificities (76.1–100% and 90.4–97.3%) when tested with serum samples from Leishmania -infected dogs and human patients with VL. Overall, no single recombinant antigen was sufficient to serodiagnosis all canine or human VL cases

Topics: Doenças do Cão/diagnóstico, Genes de Protozoários/genética, Leishmania infantum/genética, Leishmaniose Visceral/diagnóstico, Antígenos de Protozoários/genética, Animais, Clonagem Molecular, Doenças do Cão/parasitologia, Cães, Ensaio de Imunoadsorção Enzimática/métodos, Ensaio de Imunoadsorção Enzimática/veterinária, Proteínas de Choque Térmico HSP70/genética, Humanos, Cinesina/genética, Leishmaniose Visceral/parasitologia, Leishmaniose Visceral/veterinária, Poliubiquitina/genética, Proteínas Recombinantes/genética, Sensibilidade e Especificidade, Testes Sorológicos/métodos
Publisher: American Society of Tropical Medicine and Hygiene
Year: 2011
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