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Molecular epidemiology of HIV-1 in Brazil: high prevalence of HIV-1 subtype B and identification of an HIV-1 subtype D infection in the city of Rio de Janeiro, Brazil. Evandro Chagas Hospital AIDS Clinical Research Group.

By Mariza Gonçalves Morgado, Monick Lindenmeyer Guimarães, Carmen B. G Gripp, Catia Ines Costa, Ivan Neves Junior, Valdiléia Gonçalves Veloso, Maria Inês Linhares de Carvalho, Luis Roberto Ribeiro Castello-Branco, Francisco Inácio Bastos, Carla Kuiken C, Euclides A Castilho, Bernardo Galvão Castro Filho, Vera Bongertz and Evandro Chagas Hospital AIDS Clinical Research Group


HIV-1-positive individuals were recruited from January 1993 to December 1996 from several cohorts receiving follow-up in the city of Rio de Janeiro, Brazil, to evaluate HIV-1 genetic variability and the potential association with modes of transmission. HIV-1 subtyping was carried out using the heteroduplex mobility assay (HMA), and those samples corresponding to the typical Brazilian subtype B variant were further identified based on the Fok I restriction fragment length polymorphism (RFLP). DNA sequencing was performed to evaluate one case of subtype D infection. From the 131 HIV-1-positive individuals analyzed, 106 (80.9%) could be identified as infected by subtype B and 20 (15.3%) by subtype F. One of the samples (0.8%) was classified as subtype D. DNA samples from 4 patients (3.0%) did not yield polymerase chain reaction (PCR)-amplified products to be typed. Based on the Fok I RFLP, 39 of the 106 subtype B samples (37%) were identified as corresponding to the typical Brazilian subtype B variant containing the GWGR motif at the tip of the V3 loop. No statistically significant association could be detected between HIV-I subtypes and modes of transmission, exposure categories, or gender. This is the first reported case of HIV-1 subtype D infection in Brazi

Topics: HIV-1 subtypes in Brazil, Heteroduplex mobility assay, HIV-1 subtype D infection, Infecções por HIV/epidemiologia, HIV-1/classificação, Sequência de Aminoácidos, Brasil/epidemiologia, Estudos de Coortes, Sequência Consenso, DNA Viral/química, Feminino, Produtos do Gene env/química, Variação Genética, Infecções por HIV/transmissão, HIV-1/genética, Humanos, Masculino, Epidemiologia Molecular, Dados de Sequência Molecular, Ácidos Nucleicos Heteroduplexes, Filogenia, Reação em Cadeia da Polimerase, Polimorfismo de Fragmento de Restrição, Prevalência, Alinhamento de Sequência, Distribuição por Sexo, População Urbana
Publisher: Lippincott-Raven Publishers, Philadelphia
Year: 1998
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