Molecular epidemiology of HIV-1 in Brazil: high prevalence of HIV-1 subtype B and identification of an HIV-1 subtype D infection in the city of Rio de Janeiro, Brazil. Evandro Chagas Hospital AIDS Clinical Research Group.
Abstract
HIV-1-positive individuals were recruited from January 1993 to December 1996 from several cohorts receiving follow-up in the city of Rio de Janeiro, Brazil, to evaluate HIV-1 genetic variability and the potential association with modes of transmission. HIV-1 subtyping was carried out using the heteroduplex mobility assay (HMA), and those samples corresponding to the typical Brazilian subtype B variant were further identified based on the Fok I restriction fragment length polymorphism (RFLP). DNA sequencing was performed to evaluate one case of subtype D infection. From the 131 HIV-1-positive individuals analyzed, 106 (80.9%) could be identified as infected by subtype B and 20 (15.3%) by subtype F. One of the samples (0.8%) was classified as subtype D. DNA samples from 4 patients (3.0%) did not yield polymerase chain reaction (PCR)-amplified products to be typed. Based on the Fok I RFLP, 39 of the 106 subtype B samples (37%) were identified as corresponding to the typical Brazilian subtype B variant containing the GWGR motif at the tip of the V3 loop. No statistically significant association could be detected between HIV-I subtypes and modes of transmission, exposure categories, or gender. This is the first reported case of HIV-1 subtype D infection in Brazi- info:eu-repo/semantics/article
- info:eu-repo/semantics/publishedVersion
- HIV-1 subtypes in Brazil
- Heteroduplex mobility assay
- HIV-1 subtype D infection
- Infecções por HIV/epidemiologia
- HIV-1/classificação
- Sequência de Aminoácidos
- Brasil/epidemiologia
- Estudos de Coortes
- Sequência Consenso
- DNA Viral/química
- Feminino
- Produtos do Gene env/química
- Variação Genética
- Infecções por HIV/transmissão
- HIV-1/genética
- Humanos
- Masculino
- Epidemiologia Molecular
- Dados de Sequência Molecular
- Ácidos Nucleicos Heteroduplexes
- Filogenia
- Reação em Cadeia da Polimerase
- Polimorfismo de Fragmento de Restrição
- Prevalência
- Alinhamento de Sequência
- Distribuição por Sexo
- População Urbana