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Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis

By Artur F Barbosa, Bruno B Sangiorgi, Suely L Galdino, Manoel Barral Netto, Ivan R Pitta and Antonio Luiz Barbosa Pinheiro

Abstract

Cutaneous and mucocutaneous leishmaniasis are diseases characterized by skin or mucosal manifestations. In the new world, Leishmania braziliensis is the main etiological agent of cutaneous leishmaniasis, condition that may evolve to the mucocutaneous form. The therapeutic arsenal routinely employed to treat infected patients is unsatisfactory, especially for pentavalent antimonials, treatment recommended by the WHO, as they are often highly toxic, poorly tolerated and of variable effectiveness. This work aimed to evaluate in vitro the effectiveness of photodynamic antimicrobial chemotherapy as a new approach for the treatment of leishmaniasis. MATERIALS AND METHODS: A laser (λ = 660 nm, 40 mW, 4.2 J/cm2 , and 8.4 J/cm2 , CW) associated to phenothiazine's derivatives (5 and 10 µg/ml, toluidine blue O, methylene blue, or phenothiazine) on the promastigote forms of L. braziliensis in a single session. Samples were removed and analyzed in a hemocytometer 72 hours after PACT and viability of the parasites was assessed in quadruplicates. RESULTS: An important decrease in the number of viable parasites on all treated groups in comparison to their controls was observed as all tested compounds lead to significant parasite lethality being the highest lethality achieved with 10 µg/ml of TBO. No lethality was observed on groups treated with laser or with any of the compounds separately. CONCLUSIONS: TBO presented higher parasite lethality in comparison to MB with impressive reduction from 1 hour to 5 minutes of pre-incubation time

Topics: Laser-therapy, Leishmaniasis, Methylene blue, Toluidine blue O, Treatment, Leishmania braziliensis/efeitos de drogas, Leishmaniose Cutânea/quimioterapia, Azul de Metileno/uso terapêutico, Fotoquimioterapia, Fármacos Fotossensibilizantes/uso terapêutico, Cloreto de Tolônio/uso terapêutico, Tripanossomicidas/uso terapêutico, Humanos, Azul de Metileno/farmacologia, Fenotiazinas/farmacologia, Fenotiazinas/uso terapêutico, Fármacos Fotossensibilizantes/farmacologia, Cloreto de Tolônio/farmacologia, Tripanossomicidas/farmacologia
Publisher: Wiley Periodicals, Inc
Year: 2012
OAI identifier: oai:agregador.ibict.br.RI_FIOCRUZ:oai:localhost:icict/7350
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