Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
The incidence of Shigella spp. was assessed in 877 infants from the public hospital in Rond??nia (Western Amazon
region, Brazil) where Shigella represents the fourth cause of diarrhea. Twenty-five isolates were identified: 18
were Shigella flexneri, three Shigella sonnei, three Shigella boydii and one Shigella dysenteriae. With the exception
of S. dysenteriae, all Shigella spp. isolated from children with diarrhea acquired multiple antibiotic resistances.
PCR detection of ipa virulence genes and invasion assays of bloody diarrhea and fever (colitis) were compared
among 25 patients testing positive for Shigella. The ipaH and ipaBCD genes were detected in almost all isolates
and, unsurprisingly, all Shigella isolates associated with colitis were able to invade HeLa cells. This work alerts for
multiple antibiotic resistant Shigella in the region and characterizes presence of ipa virulence genes and invasion
phenotypesin dysenteric shigellosis
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