Administration oral probiotic with viable cells and inactivated in piglets

Abstract

O objetivo foi avaliar o uso oral de probiÃtico com cÃlulas viÃveis e inativadas (âpoolâ de Lactobacillus sp. e Lactobacillus plantarum de origem gastrointestinal de suÃnos), no desempenho, na microbiota intestinal e no sistema imune de leitÃes durante as fases de aleitamento e creche. Foram utilizados, na fase de aleitamento, 108 leitÃes e para a fase de creche 72 leitÃes desmamados aos 21 dias de idade, distribuÃdos em um delineamento experimental de blocos ao acaso, com trÃs tratamentos e seis repetiÃÃes. Os tratamentos foram: tratamento A: 1 ml de caldo MRS + 1 ml de soluÃÃo salina estÃril; tratamento B: 1 ml de probiÃtico (8,60 X 107 UFC/ml de um âpoolâ de Lactobacillus sp e Lactobacillus plantarum) ativadas no caldo MRS + 1 ml de soluÃÃo salina; tratamento C: 1 ml de probiÃtico contendo cÃlulas inativadas no caldo MRS + 1 ml de soluÃÃo salina. Os tratamentos foram administrados via oral aos leitÃes diariamente no perÃodo da manhÃ, do nascimento aos 35 dias de idade sendo 1 ml por animal na fase de aleitamento e 2 ml na fase de creche. As contagens de bactÃrias acidolÃticas e coliformes nÃo foram influenciadas (P>0,05) pelos tratamentos e tempos de coleta. NÃo foi observado efeito (P>0,05) dos tratamentos para ganho de peso e ganho diÃrio de peso dos leitÃes do nascimento aos 21 dias de idade. Dos 21 aos 35 dias de idade observou-se para o tratamento controle menor consumo e menor consumo diÃrio de raÃÃo (P<0,05) quando comparado aos tratamentos probiÃticos e maior ganho de peso e ganho diÃrio de peso (P<0,05) foi observado no tratamento probiÃtico com cÃlulas inativadas em relaÃÃo ao tratamento probiÃtico com cÃlulas viÃveis e o controle. Observou-se menor concentraÃÃo (P<0,05) de globulina nos tratamentos com probiÃticos em relaÃÃo ao controle. NÃo foram observadas diferenÃas significativas (P>0,05) para as variÃveis proteÃnas sÃricas totais, albumina, glicose, hemoglobina, leucÃcitos, hematÃcrito, hemÃcias, eosinÃfilos, bastÃes, segmentados, linfÃcitos, monÃcitos, plaquetas e para os nÃveis sÃricos de IgA entre os tratamentos. Concluiu-se que o uso oral de probiÃtico com cÃlulas viÃveis e inativadas nÃo alterou as contagens microbiolÃgicas, os valores das proteÃnas sÃricas totais, albumina, glicose, hemograma e IgA e uso de probiÃticos com cÃlulas viÃveis e inativadas diminuiu os nÃveis sÃricos de globulinaThe objective was to evaluate the use oral probiotic of with viable cells and inactivated ("pool" of Lactobacillus sp. and Lactobacillus plantarum of gastrointestinal origin of pigs), on performance, intestinal microbiota and the immune system of piglets during the stages of lactation and creche. Were used, 108 lactating piglets and for nursery phase 72 piglets weaned at 21 days old, divided into an experimental design of randomized blocks with three treatments and six replicates. The treatments were: Treatment A: 1 ml of MRS broth + 1 ml of sterile saline, treatment B: 1 ml of probiotic (8.60 X 107 CFU/ml of a "pool" of Lactobacillus plantarum and Lactobacillus sp) activated MRS broth + 1 ml of saline, treatment C: 1 ml of probiotic inactivated cells in MRS broth + 1 ml of saline. Treatments were administered orally to piglets in the daily morning, from birth to 35 days of age with 1 ml per animal lactating and 2 ml in the nursery phase. The count of acidolÃticas bacteria, and coliforms were not affected (P> 0.05) by treatments and sampling times. There was no effect (P>0.05) of treatments for weight gain and weight gain daily of piglets from birth to 21 days old. From 21 to 35 days of age was observed lower consumption and lower daily feed intake (P<0.05) for the control treatment when compared to the probiotic treatments and greater weight gain and weight daily gain (P<0.05) was observed in probiotic treatment with cells inactivated in relation to probiotic treatment with viable cells and control. There was less concentration (P<0.05) globulin in the treatment with probiotics compared to control. There were no significant differences (P>0.05) for the variables serum protein, albumin, glucose, hemoglobin, leukocytes, hematocrit, red blood cells, eosinophils, sticks, segments, lymphocytes, monocytes, platelets and serum levels of IgA between treatments. It was concluded that oral use of probiotics with viable cells and inactivated did not alter microbial counts, the values of serum protein, albumin, glucose, blood count and IgA and use of probiotics with viable and inactivated cells diminished serum globuli