Location of Repository

Cryobiology

By Iracema A. Nascimento, Maria Bernadete N.L. Leite, Milena Maria Sampaio de Araújo, Giovanni Sansone, Solange A. Pereira and Maristela do Espírito Santo

Abstract

Texto completo: acesso restrito. p. 113–117Cryopreservation is a valuable tool for aquaculture by providing continuous seed production, regardless of the spawning seasons. This study aimed to select the least toxic among the cryoprotectants dimethyl sulfoxide (Me2SO), propylene glycol (PG), and methanol (MET) based on their toxicological effects on Crassostrea rhizophorae gametes and trochophores. They were exposed for 10, 20, and 30 min to a range of concentrations of those cryoprotectants. The endpoint was EC15–24 h (effective concentration which causes abnormalities in 15% of the population exposed to the cryoprotectants for 24 h), recently determined as the chronic value (the concentration at which chronic effects are first observed) for C. rhizophorae embryonic phases. There were no significant differences (p > 0.05) among the exposure times in Me2SO toxic effects to either gametes or trochophores. For MET, the increase in exposure time resulted in higher toxicity for gametes, but not for trochophores, while for PG there was a significant (p > 0.05) increase in toxicity with the increase of exposure for trochophores and spermatozoa, but not for oocytes. For gametes, MET was the most toxic among the cryoprotectants, while PG was the most toxic for trochophores

Topics: Cryoprotectants, Toxic effects, Cryopreservation protocols, Oyster gametes, Trochophores
Year: 2013
OAI identifier: oai:agregador.ibict.br.RI_UFBA:oai:192.168.11:11:ri/13645
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.rcaap.pt/detail.jsp... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.