To study the prevalence of ASB in diabetic women and to compare microbiological profile among diabetic and non- diabetic women. Material and methods:- In this prospective study, 100 midstream urine samples were collected from diabetic women without any signs and symptoms of urinary tract infection. Routine standard laboratory methods were used for isolation and identification of uropathogens. Antibiotic sensitivity test was done on MHA media by using Kirby Bauers disk diffusion method. Result: Prevalence of ASB was 13% in diabetic and 6% in non-diabetic women.E-coli found to be leading pathogen among diabetic and in non-diabetic women. Nitrofurantoin and Amikacin were found to be the most effective drugs against large types of bacteria. Conclusion:- The initial choice of empirical antimicrobial therapy should be based on Gram stain and urine culture. Choice of antimicrobial therapy should integrate local sensitivity patterns of infecting organisms
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